| American Mariculture, Inc. | Agriculture, Forestry, Fishing and Hunting | $758,725 | $762,134 | 92 | $15.1M |  AI Deep Dive |
| Dynamic South INC | Construction | $451,560 | $453,564 | 26 | $8.9M | AI Deep Dive |
| Island Crab Corporation | Accommodation and Food Services | $300,405 | $302,641 | 34 | $6.0M | AI Deep Dive |
| Grace Fisheries, LLC | Agriculture, Forestry, Fishing and Hunting | $106,627 | $107,616 | 4 | <$5M | AI Deep Dive |
| Grace Fisheries LLC | Agriculture, Forestry, Fishing and Hunting | $106,627 | $107,447 | 5 | <$5M | AI Deep Dive |
| Pine Island Animal Clinic LLC | Professional, Scientific, and Technical Services | $77,085 | $77,539 | 13 | <$5M | AI Deep Dive |
| Girls5dad1 Charters LLC | Accommodation and Food Services | $68,846 | $69,236 | 10 | <$5M | AI Deep Dive |
| Beacon of Hope Pine Island INC | Other Services (except Public Administration) | $38,621 | $38,778 | 12 | <$5M | AI Deep Dive |
| 1st Enviro Safety INC | Manufacturing | $32,300 | $32,476 | 8 | $7.9M | AI Deep Dive |
| Island Lawn Care and Landscaping Company | Administrative and Support and Waste Management | $29,824 | $30,121 | 4 | <$5M | AI Deep Dive |
| Mark Massey Landscaping LLC | Administrative and Support and Waste Management | $25,837 | $26,056 | 3 | <$5M | AI Deep Dive |
| Pine Island Community Church | Other Services (except Public Administration) | $24,320 | $24,458 | 4 | N/A | AI Deep Dive |
| Romana's LLC | Accommodation and Food Services | $22,194 | $22,323 | 6 | <$5M | AI Deep Dive |
| J-Squared Productions INC | Professional, Scientific, and Technical Services | $19,792 | $19,881 | 1 | <$5M | AI Deep Dive |
| Cutthroat Clams | Agriculture, Forestry, Fishing and Hunting | $15,592 | $15,699 | 8 | <$5M | AI Deep Dive |
| Silver Lining Studios | Arts, Entertainment, and Recreation | $15,332 | $15,419 | 1 | <$5M | AI Deep Dive |
| Ted Carignan L.l.c | Manufacturing | $12,717 | $12,860 | 2 | <$5M | AI Deep Dive |
| M Shane Dooley | Transportation and Warehousing | $8,249 | $8,308 | 1 | <$5M | AI Deep Dive |
| Whitehouse Plantation LLC | Retail Trade | $7,919 | $8,018 | 5 | <$5M | AI Deep Dive |
| Pine Island Diversified Services, Inc. | Real Estate and Rental and Leasing | $7,564 | $7,616 | 2 | <$5M | AI Deep Dive |
| Island Couple Cleaning Service | Administrative and Support and Waste Management | $4,259 | $4,300 | 2 | <$5M | AI Deep Dive |
| Real Estate at Dockside Pllc | Real Estate and Rental and Leasing | $2,500 | $2,525 | 2 | <$5M | AI Deep Dive |
| Darrin T Covert | Other Services (except Public Administration) | $2,109 | $2,130 | 1 | <$5M | AI Deep Dive |
| Bruce Hanshaw | Agriculture, Forestry, Fishing and Hunting | $2,000 | $2,013 | 1 | <$5M | AI Deep Dive |