| E.a. Renfroe & Company, Inc. | Other Services (except Public Administration) | $10,000,000 | $10,121,667 | 218 | $41.6M |  Research |
| Automation Personnel Services, Inc. | Administrative and Support and Waste Management | $10,000,000 | $10,126,027 | 499 | $94.4M | Research |
| Maynard Cooper & Gale, Pc. | Professional, Scientific, and Technical Services | $10,000,000 | $10,132,329 | 465 | $113.0M | Research |
| American Family Care, Inc. | Health Care and Social Assistance | $10,000,000 | $10,108,611 | 500 | $156.9M | Research |
| Action Enterprise Holdings LLC | Transportation and Warehousing | $10,000,000 | $10,124,658 | 499 | $133.2M | Research |
| Satellites Unlimited LLC | Construction | $7,836,983 | $7,901,856 | 500 | $159.5M | Research |
| Southern Research Institute | Professional, Scientific, and Technical Services | $7,726,600 | $0 | 424 | N/A | Research |
| Security Engineers INC | Administrative and Support and Waste Management | $6,866,614 | $6,943,291 | 500 | $73.2M | Research |
| Balch & Bingham LLP | Professional, Scientific, and Technical Services | $6,857,800 | $6,939,332 | 381 | $92.6M | Research |
| J & M Tank Lines Inc. | Transportation and Warehousing | $6,420,100 | $6,503,561 | 250 | $57.0M | Research |
| Montgomery Transport, LLC | Transportation and Warehousing | $6,235,000 | $6,308,954 | 500 | $100.7M | Research |
| Associated Grocers of the South INC | Wholesale Trade | $6,227,900 | $3,247,818 | 270 | $69.5M | Research |
| Summitmedia LLC | Information | $5,468,863 | $5,532,541 | 349 | $58.6M | Research |
| Wood Fruitticher Grocery Company INC | Wholesale Trade | $5,274,700 | $5,336,531 | 377 | $97.0M | Research |
| Sirote & Permutt, PC | Professional, Scientific, and Technical Services | $5,100,000 | $3,468,683 | 346 | $84.1M | Research |
| Homeland Vinyl Products Inc. | Manufacturing | $4,869,586 | $5,006,475 | 500 | $495.9M | Research |
| Birmingham Jefferson Civic Center Authority | Arts, Entertainment, and Recreation | $4,867,687 | $4,775,943 | 238 | $62.5M | Research |
| The E Group, L.l.c. | Administrative and Support and Waste Management | $4,790,573 | $4,850,292 | 250 | $39.3M | Research |
| Eternal Word Television Network, Inc. | Information | $4,677,500 | $4,446,006 | 313 | $52.6M | Research |
| Bright Future Electric LLC | Construction | $4,529,073 | $4,582,164 | 439 | $144.4M | Research |
| Birmingham-Southern College | Educational Services | $4,407,500 | $4,459,043 | 349 | N/A | Research |
| Birmingham Fastener & Supply, Incorporated | Construction | $4,141,100 | $4,197,033 | 394 | $91.1M | Research |
| Adtrav Corporation | Administrative and Support and Waste Management | $4,023,032 | $4,065,497 | 322 | $101.8M | Research |
| Buddy Moore Trucking, INC | Transportation and Warehousing | $3,959,000 | $4,003,099 | 250 | $50.4M | Research |
| Glenwood, INC | Health Care and Social Assistance | $3,936,300 | $3,982,026 | 350 | N/A | Research |