| The Cherokee County Health Care Authority | Health Care and Social Assistance | $1,840,500 | $1,849,961 | 314 | N/A |  Research |
| Cherokee Gin & Cotton Company | Agriculture, Forestry, Fishing and Hunting | $264,055 | $266,329 | 34 | <$5M | Research |
| Cherokee Gin & Cotton Company | Agriculture, Forestry, Fishing and Hunting | $259,372 | $260,741 | 14 | <$5M | Research |
| Model Tees | Manufacturing | $258,500 | $254,092 | 43 | $42.6M | Research |
| David Crawford | Manufacturing | $252,500 | $254,296 | 43 | $42.6M | Research |
| Cherokee Farmers Cooperative, INC | Retail Trade | $234,445 | $236,430 | 28 | N/A | Research |
| Dixie Green INC | Agriculture, Forestry, Fishing and Hunting | $230,825 | $232,069 | 44 | <$5M | Research |
| Dixie Green, Inc. | Agriculture, Forestry, Fishing and Hunting | $230,800 | $232,101 | 45 | <$5M | Research |
| J & a Pharmacy INC | Retail Trade | $229,600 | $187,423 | 7 | <$5M | Research |
| Prohealth Northeast Alabama, LLC | Health Care and Social Assistance | $229,200 | $231,594 | 44 | $7.5M | Research |
| Perry Medical | Health Care and Social Assistance | $219,200 | $220,661 | 23 | $7.2M | Research |
| Triple C Development, Inc. | Real Estate and Rental and Leasing | $208,772 | $210,477 | 9 | <$5M | Research |
| Dean's Drugs, Inc. | Retail Trade | $200,000 | $201,036 | 14 | <$5M | Research |
| Triple C Development Inc. | Construction | $171,470 | $172,395 | 5 | $5.7M | Research |
| Coosa Valley Turf Farms, LLC | Agriculture, Forestry, Fishing and Hunting | $164,100 | $165,814 | 14 | <$5M | Research |
| Weiss Lake Egg Company INC | Agriculture, Forestry, Fishing and Hunting | $156,400 | $94,399 | 16 | <$5M | Research |
| Cherokee Community Dialysis, LLC | Health Care and Social Assistance | $154,322 | $155,266 | 15 | <$5M | Research |
| Preferred Health Services, Inc. | Administrative and Support and Waste Management | $150,200 | $151,185 | 9 | <$5M | Research |
| Foothills Enterprises, LLC | Retail Trade | $150,000 | $151,129 | 15 | <$5M | Research |
| Cherokee Eye Clinic, PC | Health Care and Social Assistance | $129,000 | $130,672 | 10 | <$5M | Research |
| Cherokee Pain Management, LLC | Health Care and Social Assistance | $114,796 | $115,903 | 10 | <$5M | Research |
| Mach-1 Express Wash, LLC | Other Services (except Public Administration) | $114,285 | $115,004 | 24 | <$5M | Research |
| Pruitt & Eberhart, Dmd, P.c. | Health Care and Social Assistance | $110,794 | $111,431 | 12 | <$5M | Research |
| Pruitt & Eberhart, Dmd, P.c. | Health Care and Social Assistance | $110,794 | $111,492 | 12 | <$5M | Research |
| Coffey Agencies | Finance and Insurance | $107,000 | $108,858 | 13 | $6.7M | Research |