| Dothan Security INC | Administrative and Support and Waste Management | $10,000,000 | $10,141,111 | 500 | $73.2M |  Research |
| Sunsouth, LLC | Wholesale Trade | $4,169,000 | $4,221,460 | 465 | $142.4M | Research |
| Seven Restaurants, LLC | Accommodation and Food Services | $4,130,980 | $4,179,194 | 500 | $88.8M | Research |
| Four Star Freightliner, Inc. | Wholesale Trade | $2,573,167 | $2,602,706 | 200 | $61.3M | Research |
| Bondy's Ford INC | Retail Trade | $2,362,000 | $2,419,016 | 223 | $56.2M | Research |
| Spectracare Health Systems, Inc. | Health Care and Social Assistance | $2,300,000 | $2,327,408 | 302 | N/A | Research |
| Forster & Howell, INC | Retail Trade | $2,189,687 | $2,215,659 | 445 | $111.1M | Research |
| Eye Partners, P.c. | Health Care and Social Assistance | $2,177,025 | $2,201,819 | 243 | $56.9M | Research |
| Eye Partners P.c. | Health Care and Social Assistance | $2,000,000 | $2,007,562 | 293 | $92.0M | Research |
| Dothan Pediatric Clinic PA | Health Care and Social Assistance | $1,989,355 | $2,003,471 | 183 | $57.4M | Research |
| Sbjs PC | Health Care and Social Assistance | $1,917,845 | $1,939,154 | 161 | $50.5M | Research |
| Extendicare, Inc. | Health Care and Social Assistance | $1,796,512 | $1,814,526 | 290 | $49.3M | Research |
| Dothan Pediatric Clinic P.a. | Health Care and Social Assistance | $1,750,290 | $1,761,655 | 172 | $54.0M | Research |
| Mark Dunning Industries Inc. | Administrative and Support and Waste Management | $1,699,200 | $1,711,991 | 160 | $30.3M | Research |
| Dunbarton Corporation | Manufacturing | $1,610,700 | $1,622,350 | 160 | $39.1M | Research |
| Kfh Industries, Inc. | Manufacturing | $1,490,047 | $1,506,131 | 159 | $157.7M | Research |
| Gut P.c. | Health Care and Social Assistance | $1,431,610 | $1,439,768 | 106 | $33.3M | Research |
| Larry Blumberg and Associates, Inc. | Real Estate and Rental and Leasing | $1,296,803 | $1,304,832 | 78 | $11.7M | Research |
| Larry Blumberg and Associates, Inc. | Real Estate and Rental and Leasing | $1,296,800 | $1,308,740 | 78 | $11.7M | Research |
| Commercial Jet Services LLC | Manufacturing | $1,198,200 | $1,208,803 | 82 | $21.5M | Research |
| Wall Street Markets, LLC | Retail Trade | $1,108,900 | $1,116,070 | 273 | $91.4M | Research |
| Professional Resource Management of Wiregrass, LLC | Administrative and Support and Waste Management | $1,108,787 | $1,122,154 | 44 | $6.9M | Research |
| Mike Schmitz Automotive Group, Inc. | Retail Trade | $1,011,700 | $1,017,883 | 100 | $25.2M | Research |
| Smith's Inc. of Dothan | Construction | $999,646 | $1,004,922 | 82 | $25.9M | Research |
| Jeffers INC | Retail Trade | $992,900 | $1,000,099 | 142 | $22.2M | Research |