| Cates & Puckett Construction Company Inc. | Construction | $413,782 | $416,506 | 55 | $62.7M |  Research |
| Northwest Alabama Cancer Center, P.c. | Health Care and Social Assistance | $398,337 | $404,002 | 28 | $5.6M | Research |
| Ironman Pizza, INC | Accommodation and Food Services | $382,650 | $385,116 | 111 | $19.7M | Research |
| Blake Freeman Inc. | Transportation and Warehousing | $374,435 | $376,526 | 47 | $11.0M | Research |
| United Treating and Distribution | Wholesale Trade | $358,302 | $360,322 | 24 | $7.4M | Research |
| Lewis Electric Supply Co., INC | Wholesale Trade | $337,303 | $339,541 | 25 | $7.7M | Research |
| Freeman & Freeman Inc. | Transportation and Warehousing | $332,777 | $335,874 | 45 | $10.5M | Research |
| Lewis Electric Supply Co. INC | Wholesale Trade | $328,757 | $331,252 | 25 | $7.7M | Research |
| Grace Life of the Shoals | Other Services (except Public Administration) | $303,600 | $0 | 24 | N/A | Research |
| Dynamic Dentistry of the Shoals PC | Health Care and Social Assistance | $301,169 | $302,307 | 30 | $7.0M | Research |
| M G Roofing INC | Construction | $260,262 | $261,513 | 22 | $7.3M | Research |
| Civil Group, LLC | Professional, Scientific, and Technical Services | $254,935 | $256,592 | 17 | <$5M | Research |
| Kentucky Fried Chicken of Colbert, INC | Accommodation and Food Services | $245,800 | $247,657 | 69 | $12.3M | Research |
| Anesthesia Medical Consultants, LLC | Health Care and Social Assistance | $241,785 | $359,286 | 5 | <$5M | Research |
| D & D Total Health Solutions, Inc. | Retail Trade | $228,400 | $230,050 | 21 | <$5M | Research |
| M G Roofing INC | Construction | $226,298 | $227,626 | 25 | $8.2M | Research |
| Dynamic Dentistry of the Shoals, P.c. | Health Care and Social Assistance | $226,045 | $227,765 | 22 | $5.1M | Research |
| James A. Ryerson, Dmd, P.c. | Health Care and Social Assistance | $218,065 | $219,888 | 24 | $5.6M | Research |
| James a Ryerson Dmd PC | Health Care and Social Assistance | $218,065 | $219,307 | 24 | $5.6M | Research |
| Kl Arnold Enterprises Inc. | Retail Trade | $213,440 | $214,780 | 18 | <$5M | Research |
| United Wood Preserving LLC | Manufacturing | $212,517 | $213,833 | 27 | $26.8M | Research |
| Farris Wholesale Outlet, INC | Wholesale Trade | $208,916 | $211,342 | 20 | $6.1M | Research |
| Tri Cities Truck Parts INC | Wholesale Trade | $189,037 | $190,429 | 12 | <$5M | Research |
| Highland Park Baptist Church | Other Services (except Public Administration) | $185,621 | $186,327 | 20 | N/A | Research |
| Dr. Ly Natural Health Inc. | Health Care and Social Assistance | $178,575 | $179,157 | 18 | <$5M | Research |