| Ttl, Inc. | Professional, Scientific, and Technical Services | $4,705,490 | $4,760,780 | 349 | $70.4M |  Research |
| Sealy Management Company, Inc. | Real Estate and Rental and Leasing | $2,998,436 | $2,784,887 | 312 | $36.4M | Research |
| S. T. Bunn Construction Company, INC | Construction | $2,718,431 | $2,750,523 | 239 | $81.4M | Research |
| Southern Heat Exchanger Corp. | Management of Companies and Enterprises | $2,528,700 | $2,557,780 | 173 | $28.4M | Research |
| Boone Newspapers, Inc. | Information | $2,280,300 | $2,311,844 | 297 | $49.9M | Research |
| Northstar Ems, Inc. | Health Care and Social Assistance | $2,038,000 | $2,062,230 | 208 | $48.2M | Research |
| Southern Heat Exchanger Corporation | Manufacturing | $2,000,000 | $2,011,833 | 164 | $40.1M | Research |
| Boone Newspapers Inc. | Information | $1,898,567 | $1,825,142 | 249 | $41.8M | Research |
| Premier Service Co., Inc. | Construction | $1,725,100 | $1,742,878 | 188 | $61.9M | Research |
| Mental Health Board of Bibb, Pickens and Tuscaloosa Counties | Health Care and Social Assistance | $1,715,760 | $1,729,486 | 216 | N/A | Research |
| Morrow Realty Co., Inc. | Real Estate and Rental and Leasing | $1,657,000 | $1,288,913 | 169 | $25.4M | Research |
| Alabama Cancer Care Holdings, LLC | Finance and Insurance | $1,650,200 | $1,665,464 | 140 | $71.9M | Research |
| Stillman College | Educational Services | $1,627,948 | $1,643,142 | 153 | N/A | Research |
| Tuscaloosa County Park and Recreation Authority | Public Administration | $1,590,200 | $1,602,701 | 146 | N/A | Research |
| Stillman College | Educational Services | $1,426,300 | $1,440,088 | 224 | N/A | Research |
| The Radiology Clinic, LLC | Health Care and Social Assistance | $1,404,300 | $1,413,350 | 83 | $26.1M | Research |
| Hinton Lumber Products, Inc. | Wholesale Trade | $1,288,247 | $1,305,137 | 150 | $46.0M | Research |
| Dixie Mechanical Inc. | Professional, Scientific, and Technical Services | $1,278,900 | $1,288,421 | 80 | $9.9M | Research |
| Dixie Mechanical | Construction | $1,278,900 | $1,287,106 | 72 | $27.0M | Research |
| Jamison Money Farmer, PC | Professional, Scientific, and Technical Services | $1,243,605 | $1,259,392 | 65 | $20.7M | Research |
| Swj Technology, LLC | Mining, Quarrying, and Oil and Gas Extraction | $1,216,000 | $1,229,545 | 101 | $6.1M | Research |
| R.l. Zeigler Company, Inc. | Manufacturing | $1,213,526 | $1,229,336 | 143 | $50.5M | Research |
| University Orthopaedic Clinic, P.c. | Health Care and Social Assistance | $1,193,000 | $1,199,528 | 94 | $29.5M | Research |
| Bama Concrete Products Co., Inc. DBA Bama Concrete Products | Manufacturing | $1,185,500 | $1,194,292 | 101 | $100.2M | Research |
| Tuscaloosa Hyundai Inc. | Retail Trade | $1,153,700 | $1,164,468 | 91 | $22.9M | Research |