| Lifelong Medical Care | Health Care and Social Assistance | $9,482,635 | $9,549,682 | 500 | $89.0M |  Research |
| Berkeley Cement, Inc. | Construction | $7,912,987 | $7,975,412 | 363 | $84.0M | Research |
| O.c. Jones & Sons, Inc. | Professional, Scientific, and Technical Services | $7,013,622 | $7,102,266 | 242 | $56.1M | Research |
| Ovid Research Company | Professional, Scientific, and Technical Services | $5,911,500 | $59,491 | 4 | <$5M | Research |
| Meyer Sound Laboratories Incorporated | Information | $5,483,800 | $5,546,601 | 357 | $60.0M | Research |
| Backroads | Administrative and Support and Waste Management | $5,266,694 | $5,334,367 | 478 | $151.2M | Research |
| Moore Iacofano Goltsman Incorp | Professional, Scientific, and Technical Services | $4,617,927 | $4,670,905 | 254 | $78.7M | Research |
| Technical Safety Services, Inc. | Administrative and Support and Waste Management | $3,906,010 | $3,955,161 | 475 | $89.9M | Research |
| Crossroads Trading Co., Inc. | Retail Trade | $3,091,400 | $3,132,054 | 313 | $48.9M | Research |
| Doughtronics, INC | Wholesale Trade | $2,972,610 | $3,005,639 | 260 | $66.9M | Research |
| Andrew M Jordan, INC | Construction | $2,766,222 | $2,797,901 | 145 | $44.9M | Research |
| Ashby Lumber | Retail Trade | $2,495,600 | $2,524,180 | 158 | $32.6M | Research |
| International Child Resource Exchange Institite | Other Services (except Public Administration) | $2,159,928 | $2,186,143 | 144 | $27.5M | Research |
| Backroads | Administrative and Support and Waste Management | $2,000,000 | $2,014,247 | 252 | $79.7M | Research |
| Meyer Sound Laboratories Incorporated | Information | $2,000,000 | $2,006,575 | 174 | $29.2M | Research |
| Doughtronics INC | Wholesale Trade | $2,000,000 | $2,012,000 | 261 | $67.2M | Research |
| Pagnol Et Cie INC | Accommodation and Food Services | $1,977,241 | $1,993,384 | 130 | $23.1M | Research |
| The Berkeley Repertory Theatre Inc. | Arts, Entertainment, and Recreation | $1,837,877 | $1,851,624 | 169 | N/A | Research |
| Tippett Studio INC | Manufacturing | $1,810,540 | $1,852,505 | 88 | $23.0M | Research |
| Tippett Studio INC | Information | $1,810,540 | $1,846,751 | 101 | $17.0M | Research |
| L.j. Kruse Co. | Construction | $1,784,987 | $1,790,589 | 53 | $16.8M | Research |
| Saint Mary's College High School of Berkeley | Educational Services | $1,750,000 | $1,618,816 | 80 | N/A | Research |
| L.j. Kruse Co. | Construction | $1,703,045 | $1,721,211 | 60 | $19.0M | Research |
| Arboricultural Specialties Inc. | Administrative and Support and Waste Management | $1,692,042 | $1,710,372 | 157 | $34.0M | Research |
| Far West Brewing LLC | Accommodation and Food Services | $1,678,145 | $1,686,629 | 119 | $14.6M | Research |