| American College of Cardiology Foundation | Other Services (except Public Administration) | $10,000,000 | $10,115,278 | 465 | N/A |  Research |
| Cava Mezze Grill, LLC | Accommodation and Food Services | $10,000,000 | $10,116,389 | 500 | $88.8M | Research |
| William C. Smith & Co. Inc. | Real Estate and Rental and Leasing | $9,026,082 | $9,182,343 | 500 | $75.0M | Research |
| Wiley Rein LLP | Professional, Scientific, and Technical Services | $8,915,913 | $9,053,194 | 493 | $119.8M | Research |
| American Association for the Advancement of Science | Other Services (except Public Administration) | $8,551,640 | $1,249,186 | 414 | N/A | Research |
| National Committee for Quality Assurance | Other Services (except Public Administration) | $8,202,873 | $8,309,511 | 345 | N/A | Research |
| Fiscalnote Holdings, Inc. | Professional, Scientific, and Technical Services | $8,000,000 | $7,807,136 | 408 | $57.1M | Research |
| Omni Shoreham Corporation | Accommodation and Food Services | $5,774,210 | $2,703,985 | 486 | $152.0M | Research |
| Creative Associates International, Inc. | Professional, Scientific, and Technical Services | $5,581,000 | $5,645,026 | 500 | $51.8M | Research |
| Bates White, LLC | Professional, Scientific, and Technical Services | $5,579,500 | $5,652,498 | 227 | $23.5M | Research |
| Apco Worldwide LLC | Professional, Scientific, and Technical Services | $5,575,300 | $5,640,655 | 316 | $32.7M | Research |
| R&r Mechanical LLC | Construction | $5,353,200 | $0 | 227 | $71.8M | Research |
| Madison Marquette Realty Group | Real Estate and Rental and Leasing | $5,336,510 | $5,431,233 | 290 | $43.5M | Research |
| Annchor Construction Corporation | Construction | $5,289,199 | $5,348,467 | 386 | $439.8M | Research |
| Washington Convention and Sports Authority | Administrative and Support and Waste Management | $5,250,000 | $5,286,792 | 364 | $115.1M | Research |
| Sidwell Friends School | Educational Services | $5,219,200 | $4,824,191 | 338 | N/A | Research |
| Veracity Engineering | Professional, Scientific, and Technical Services | $5,110,900 | $0 | 110 | $22.7M | Research |
| Willard Associates, LP | Accommodation and Food Services | $4,812,642 | $4,875,340 | 314 | $98.2M | Research |
| John I Haas INC | Agriculture, Forestry, Fishing and Hunting | $4,739,270 | $4,796,141 | 456 | $22.9M | Research |
| Studios Architecture | Professional, Scientific, and Technical Services | $4,674,900 | $4,728,661 | 230 | $71.3M | Research |
| US Green Building Council INC | Other Services (except Public Administration) | $4,629,762 | $4,680,880 | 214 | N/A | Research |
| Global Tapas LLC | Accommodation and Food Services | $4,605,194 | $4,598,830 | 500 | $88.8M | Research |
| Whitman-Walker Clinic, Inc. | Health Care and Social Assistance | $4,599,695 | $4,651,569 | 306 | N/A | Research |
| Atlas Research LLC | Professional, Scientific, and Technical Services | $4,524,800 | $4,576,584 | 193 | $20.0M | Research |
| Center for Strategic and International Studies, INC | Professional, Scientific, and Technical Services | $4,508,728 | $3,633,829 | 299 | N/A | Research |