| Dewar Nurseries, INC | Agriculture, Forestry, Fishing and Hunting | $2,761,200 | $2,792,494 | 279 | $28.5M |  Research |
| Orlando Freightliner, Inc. | Wholesale Trade | $2,194,200 | $2,220,170 | 176 | $53.9M | Research |
| Mullinax Ford of Central Florida | Retail Trade | $2,181,900 | $2,206,289 | 184 | $28.7M | Research |
| Central Florida Hospitalist Partners | Health Care and Social Assistance | $1,933,200 | $1,959,153 | 101 | $31.7M | Research |
| Seretta Construction Inc. | Construction | $1,889,483 | $1,905,229 | 167 | $38.6M | Research |
| Carter Electric, Inc. | Construction | $1,821,800 | $1,840,717 | 132 | $43.4M | Research |
| Seretta Construction, Inc. | Construction | $1,688,800 | $1,700,575 | 162 | $37.5M | Research |
| Mobile Mix Masters, Inc. | Construction | $1,527,600 | $1,539,402 | 155 | $35.9M | Research |
| Collins Mfg., Inc. | Manufacturing | $1,236,400 | $1,243,405 | 96 | $25.1M | Research |
| Florida Utility Trailers INC | Manufacturing | $1,142,470 | $1,148,323 | 104 | $27.2M | Research |
| Spegal Plumbing LLC | Construction | $1,094,405 | $1,100,672 | 91 | $28.8M | Research |
| Florida Restaurant Operations Group INC | Accommodation and Food Services | $1,091,170 | $1,097,687 | 197 | $35.0M | Research |
| Kenpat (central Florida) LLC | Construction | $1,026,460 | $1,033,462 | 81 | $20.5M | Research |
| Raynor Shine Services, LLC | Wholesale Trade | $998,330 | $0 | 90 | $27.6M | Research |
| Environmental Products Group, Inc. | Retail Trade | $991,900 | $999,394 | 72 | $18.8M | Research |
| Kenpat (central Florida), LLC | Construction | $958,000 | $965,638 | 80 | $20.2M | Research |
| Deroose Plants Inc. | Agriculture, Forestry, Fishing and Hunting | $945,254 | $952,376 | 152 | $15.5M | Research |
| Livetrends Design Group, LLC | Wholesale Trade | $932,900 | $939,878 | 177 | $54.2M | Research |
| Entertainment Retail Enterprises, LLC | Wholesale Trade | $921,000 | $933,187 | 98 | $25.2M | Research |
| Entertainment Retail Enterprises LLC | Manufacturing | $920,882 | $930,833 | 92 | $25.7M | Research |
| Honey Transport, INC | Transportation and Warehousing | $898,707 | $907,594 | 93 | $14.1M | Research |
| Florida Restaurant Operations Group INC | Retail Trade | $811,100 | $818,367 | 197 | $21.9M | Research |
| A & B Electric Co., Inc. | Construction | $782,860 | $793,081 | 99 | $32.6M | Research |
| Gellner Enterprises LLC | Administrative and Support and Waste Management | $774,716 | $605,005 | 165 | $35.7M | Research |
| Agri-Starts, Inc. | Agriculture, Forestry, Fishing and Hunting | $754,500 | $758,859 | 120 | $12.3M | Research |