| Desoto County Hospital District | Health Care and Social Assistance | $2,700,000 | $2,730,403 | 308 | N/A |  Research |
| A+ Environmental Restoration, LLC | Administrative and Support and Waste Management | $1,327,285 | $1,337,799 | 93 | $20.1M | Research |
| Hb Harvesting INC | Agriculture, Forestry, Fishing and Hunting | $1,250,000 | $457,098 | 133 | $6.7M | Research |
| Bethel Farms, Lllp | Agriculture, Forestry, Fishing and Hunting | $998,327 | $1,008,556 | 107 | $17.5M | Research |
| Sun Bulb Company | Agriculture, Forestry, Fishing and Hunting | $993,180 | $1,004,519 | 50 | $5.1M | Research |
| Cutting Edge Pizza INC | Accommodation and Food Services | $854,900 | $859,819 | 226 | $40.1M | Research |
| Crown Building Products of Florida, LLC | Manufacturing | $793,232 | $800,358 | 42 | $41.7M | Research |
| Desoto Automotive Enterprises, INC | Retail Trade | $787,947 | $794,141 | 70 | $17.6M | Research |
| Crisp Citrus Harvesting LLC | Agriculture, Forestry, Fishing and Hunting | $729,302 | $734,917 | 416 | $20.9M | Research |
| Kingseal LLC DBA Desoto Health and Rehab | Health Care and Social Assistance | $524,400 | $529,076 | 98 | $16.7M | Research |
| Sandhill Environmental Services LLC | Agriculture, Forestry, Fishing and Hunting | $451,000 | $454,119 | 45 | <$5M | Research |
| Sandhill Environmental Services LLC | Administrative and Support and Waste Management | $451,000 | $454,320 | 46 | $10.0M | Research |
| Other Side Sod Company, LLC | Agriculture, Forestry, Fishing and Hunting | $446,605 | $451,803 | 61 | $6.2M | Research |
| J J C Enterprises INC | Agriculture, Forestry, Fishing and Hunting | $403,938 | $405,753 | 80 | <$5M | Research |
| Vowels, Inc. Mcdonald's | Accommodation and Food Services | $327,500 | $330,685 | 160 | $28.4M | Research |
| Harplyn INC | Utilities | $316,808 | $318,955 | 40 | N/A | Research |
| Harplyn INC | Agriculture, Forestry, Fishing and Hunting | $300,000 | $334,501 | 29 | <$5M | Research |
| Agriscapes LLC | Administrative and Support and Waste Management | $296,120 | $298,692 | 39 | $8.4M | Research |
| Agriscapes LLC | Administrative and Support and Waste Management | $296,120 | $297,434 | 35 | $7.6M | Research |
| Womack Sanitation INC | Administrative and Support and Waste Management | $248,035 | $250,617 | 22 | <$5M | Research |
| Vch Management, INC | Agriculture, Forestry, Fishing and Hunting | $224,037 | $225,375 | 34 | <$5M | Research |
| Cooper Timber Harvesting Inc. | Agriculture, Forestry, Fishing and Hunting | $210,920 | $212,550 | 18 | <$5M | Research |
| Cooper Timber Harvesting, Inc. | Agriculture, Forestry, Fishing and Hunting | $210,900 | $213,044 | 23 | <$5M | Research |
| Perez Trucking & Sod Inc. | Transportation and Warehousing | $199,127 | $200,038 | 27 | <$5M | Research |
| Eugene H. Turner & Son, Inc. | Agriculture, Forestry, Fishing and Hunting | $197,500 | $0 | 34 | <$5M | Research |