| Bynum Transport INC | Transportation and Warehousing | $2,806,300 | $2,837,949 | 275 | $55.4M |  Research |
| Hands On Staffing Payroll, INC | Administrative and Support and Waste Management | $1,206,659 | $1,220,167 | 88 | $13.8M | Research |
| Hands On Staffing Payroll Inc. | Administrative and Support and Waste Management | $1,080,157 | $1,086,278 | 73 | $11.5M | Research |
| Berkley Charter Elementary Sch | Educational Services | $1,033,000 | $1,038,136 | 108 | $13.0M | Research |
| Auburndale Oaks Care Acquisition, LLC | Health Care and Social Assistance | $989,187 | $997,458 | 141 | $24.0M | Research |
| Hands On Staffing of Central Florida, Inc. | Administrative and Support and Waste Management | $812,223 | $819,736 | 147 | $23.1M | Research |
| All Pro Auto Parts, Inc. | Retail Trade | $720,915 | $725,261 | 72 | $19.5M | Research |
| Hr Payroll Services INC | Administrative and Support and Waste Management | $718,855 | $724,586 | 171 | $26.9M | Research |
| Hands On Staffing of Central Florida INC | Professional, Scientific, and Technical Services | $615,877 | $618,392 | 100 | $14.0M | Research |
| Mulling Insurance Agency INC | Finance and Insurance | $563,700 | $569,102 | 49 | $25.2M | Research |
| Hands On Staffing of Jacksonville INC | Administrative and Support and Waste Management | $562,459 | $566,662 | 85 | $13.4M | Research |
| The Florida Brewery, Inc. | Manufacturing | $504,343 | $506,893 | 48 | $16.9M | Research |
| Hands On Staffing of Jacksonville Inc. | Administrative and Support and Waste Management | $472,991 | $476,289 | 79 | $12.4M | Research |
| Fi-Foil Company, Inc. | Manufacturing | $454,192 | $457,589 | 30 | $7.3M | Research |
| Imperial Paving LLC | Construction | $451,798 | $457,145 | 39 | $12.1M | Research |
| Harding Bell International, INC | Professional, Scientific, and Technical Services | $450,773 | $453,240 | 44 | $14.0M | Research |
| Adams Cold Storage, LLC | Transportation and Warehousing | $399,500 | $401,853 | 41 | $10.4M | Research |
| Tri Florida Water Treatment INC | Retail Trade | $357,165 | $359,080 | 44 | $6.9M | Research |
| Integrity Homes & Construction INC | Construction | $332,500 | $335,012 | 24 | $7.3M | Research |
| Kesco, Inc. | Retail Trade | $318,430 | $320,845 | 65 | $11.8M | Research |
| Smithbilt Industries, Inc. | Manufacturing | $274,450 | $279,181 | 45 | $11.0M | Research |
| New Life Facility Services, Inc. | Administrative and Support and Waste Management | $258,000 | $259,799 | 50 | $10.8M | Research |
| Springer Brothers Air Conditioning and Heating, LLC | Construction | $251,400 | $253,209 | 22 | $7.0M | Research |
| Universal Service Holding Corp | Construction | $249,800 | $251,194 | 29 | $33.0M | Research |
| Terrasouth Environmental LLC | Administrative and Support and Waste Management | $227,050 | $228,892 | 24 | $5.2M | Research |