| Glase Golf INC | Construction | $529,505 | $536,613 | 16 | $5.5M | Research |
| Pelican Landing Dental PA | Health Care and Social Assistance | $529,200 | $532,317 | 32 | $7.4M | Research |
| Glase Golf INC | Construction | $522,399 | $528,696 | 44 | $13.6M | Research |
| Karezi INC | Accommodation and Food Services | $512,500 | $518,593 | 32 | $5.7M | Research |
| Customer First, Inc. of Naples | Manufacturing | $511,381 | $514,898 | 42 | $41.7M | Research |
| Customer First INC of Naples | Manufacturing | $511,381 | $515,893 | 38 | $37.7M | Research |
| Collins & Dupont Interiors INC | Professional, Scientific, and Technical Services | $508,120 | $510,985 | 37 | $8.6M | Research |
| Collins & Dupont Interiors INC | Professional, Scientific, and Technical Services | $508,119 | $512,791 | 37 | $8.6M | Research |
| Food Innovations Inc. | Wholesale Trade | $502,670 | $506,161 | 26 | $6.7M | Research |
| Island Endeavors Inc. | Accommodation and Food Services | $495,470 | $497,837 | 53 | $9.4M | Research |
| Pinchers Crab Shack of Tarpon Pt. INC | Accommodation and Food Services | $494,578 | $497,627 | 75 | $13.3M | Research |
| Executive Healthcare Solutions, LLC | Health Care and Social Assistance | $493,900 | $498,081 | 98 | $15.9M | Research |
| Wireless Buybacks LLC | Retail Trade | $489,311 | $492,367 | 62 | $19.0M | Research |
| Smartadvocate LLC | Professional, Scientific, and Technical Services | $488,900 | $492,128 | 23 | $5.6M | Research |
| Phelan Holdings INC | Accommodation and Food Services | $473,025 | $477,289 | 55 | $10.3M | Research |
| Gilbert Anuez INC | Construction | $465,900 | $469,091 | 37 | <$5M | Research |
| Pinchers of Bonita Crossings INC | Accommodation and Food Services | $457,272 | $459,915 | 59 | $10.5M | Research |
| E2comply LLC | Wholesale Trade | $446,017 | $447,962 | 25 | $7.7M | Research |
| Surf 9, LLC | Wholesale Trade | $435,575 | $441,243 | 24 | $7.4M | Research |
| Advantage Plastering & Finish Carpentry, INC | Construction | $428,882 | $431,222 | 39 | $12.3M | Research |
| Oak Creek Charter School of Bonita Springs | Educational Services | $424,780 | $429,736 | 48 | N/A | Research |
| Allstate Construction INC | Construction | $423,100 | $426,555 | 34 | $38.7M | Research |
| Skillets Holdings LLC | Accommodation and Food Services | $419,539 | $421,114 | 268 | $47.6M | Research |
| Expert Lawn Care LLC | Administrative and Support and Waste Management | $407,910 | $411,229 | 47 | $10.2M | Research |
| Gulf Breeze Management Services of S.w. Florida, Inc. | Real Estate and Rental and Leasing | $407,107 | $410,680 | 33 | <$5M | Research |