| Ultimate Storage | Transportation and Warehousing | $245,700 | $248,034 | 32 | $8.0M | AI Deep Dive |
| Lyndsey Madison Corporation | Real Estate and Rental and Leasing | $245,450 | $247,723 | 18 | <$5M | AI Deep Dive |
| R&j Coatings and Waterproofing INC | Construction | $241,953 | $243,256 | 25 | $5.8M | AI Deep Dive |
| Tiffany and Associates INC | Manufacturing | $240,520 | $241,976 | 21 | $20.8M | AI Deep Dive |
| Tiffany and Associates INC DBA Independent Printing | Manufacturing | $240,520 | $242,925 | 26 | $25.8M | AI Deep Dive |
| Highlander Corp | Real Estate and Rental and Leasing | $240,178 | $241,398 | 14 | <$5M | AI Deep Dive |
| Med One Shuttle INC | Transportation and Warehousing | $239,842 | $241,945 | 32 | $9.8M | AI Deep Dive |
| Museum of Arts & Sciences, Inc. | Arts, Entertainment, and Recreation | $239,080 | $240,793 | 48 | N/A | AI Deep Dive |
| Xinim Corporation | Retail Trade | $238,600 | $221,736 | 28 | <$5M | AI Deep Dive |
| Alvarez Restaurant Holdings LLC | Accommodation and Food Services | $237,080 | $238,411 | 30 | $5.3M | AI Deep Dive |
| Florida Supply & Cleaning Products, LLC | Wholesale Trade | $236,392 | $238,966 | 20 | $5.1M | AI Deep Dive |
| Hir Db Opco LLC | Accommodation and Food Services | $236,195 | $238,518 | 43 | $13.4M | AI Deep Dive |
| Daytona Flea & Farmers Market Inc. | Retail Trade | $234,155 | $235,736 | 42 | $6.6M | AI Deep Dive |
| Dental Creations of Daytona Beach, Plc | Health Care and Social Assistance | $221,800 | $223,757 | 16 | <$5M | AI Deep Dive |
| Orlando Rentco LLC | Real Estate and Rental and Leasing | $220,437 | $0 | 44 | $19.9M | AI Deep Dive |
| Volusia Traders INC | Retail Trade | $219,790 | $221,386 | 24 | $6.0M | AI Deep Dive |
| Stonewood Brandon LLC | Accommodation and Food Services | $219,608 | $221,474 | 47 | $8.3M | AI Deep Dive |
| Atlantic Central Enterprises INC | Manufacturing | $218,580 | $221,258 | 24 | $5.9M | AI Deep Dive |
| Dme Holdings LLC | Real Estate and Rental and Leasing | $218,070 | $219,629 | 27 | <$5M | AI Deep Dive |
| Sj Moving & Storage, Inc. | Transportation and Warehousing | $216,303 | $219,277 | 30 | $7.5M | AI Deep Dive |
| Volusia Orthopaedic Trauma Call Associates, LLC | Health Care and Social Assistance | $215,300 | $216,857 | 31 | $9.7M | AI Deep Dive |
| Pouchfill Packaging | Administrative and Support and Waste Management | $214,958 | $0 | 33 | $6.2M | AI Deep Dive |
| Weston Coatings Group, Inc. | Construction | $214,500 | $216,204 | 25 | $6.3M | AI Deep Dive |
| Coleman Goodemote Construction Company, Inc. | Construction | $214,100 | $215,807 | 13 | $14.8M | AI Deep Dive |
| Ross Enterprise of Daytona Inc. | Retail Trade | $212,500 | $44,947 | 30 | <$5M | AI Deep Dive |