| Chalfont & Associates Group, INC | Accommodation and Food Services | $1,415,422 | $1,429,460 | 500 | $88.8M | Research |
| Ruleme Center LLC DBA Ruleme Center | Health Care and Social Assistance | $1,143,960 | $1,153,302 | 103 | $17.5M | Research |
| Commercial Companies INC | Construction | $1,065,000 | $855,779 | 113 | $38.5M | Research |
| Bayview Center LLC DBA Bayview Center | Health Care and Social Assistance | $936,577 | $944,564 | 109 | $18.5M | Research |
| Commercial Companies Inc. | Administrative and Support and Waste Management | $858,800 | $863,261 | 114 | $24.7M | Research |
| Liner Source INC | Wholesale Trade | $835,717 | $839,524 | 141 | $36.3M | Research |
| Liner Source, INC | Wholesale Trade | $835,717 | $840,383 | 141 | $36.3M | Research |
| Service Trucking, Inc. | Transportation and Warehousing | $809,700 | $823,964 | 71 | $14.3M | Research |
| Vann Gannaway Chevrolet, Inc. | Retail Trade | $806,400 | $814,133 | 63 | $15.9M | Research |
| Jon's Nursery | Administrative and Support and Waste Management | $776,901 | $782,669 | 105 | $22.7M | Research |
| Mid Florida Pathology, LLC | Health Care and Social Assistance | $660,200 | $664,088 | 50 | $11.6M | Research |
| Glem Health INC D/b/a Right at Home | Health Care and Social Assistance | $650,000 | $658,511 | 120 | $27.8M | Research |
| Bay Pharnacy, Inc. | Retail Trade | $509,409 | $515,069 | 46 | $8.0M | Research |
| The Boggy Creek Gang Inc. | Health Care and Social Assistance | $498,800 | $503,192 | 32 | N/A | Research |
| The Boggy Creek Gang Inc. | Health Care and Social Assistance | $411,790 | $413,677 | 30 | N/A | Research |
| Ag 3, Inc. | Retail Trade | $316,200 | $318,027 | 50 | $10.3M | Research |
| El Shaddai Pentecostal Holiness Church Inc. | Other Services (except Public Administration) | $306,512 | $138,009 | 18 | <$5M | Research |
| Faith Lutheran Church at Eustis, Florida | Other Services (except Public Administration) | $300,537 | $302,324 | 42 | N/A | Research |
| Faith Lutheran Church at Eustis, Florida | Other Services (except Public Administration) | $300,537 | $302,298 | 42 | N/A | Research |
| Bowen & Schroth P.a. | Professional, Scientific, and Technical Services | $300,000 | $302,050 | 21 | $5.1M | Research |
| Tiger Foods, Inc. | Accommodation and Food Services | $292,341 | $224,060 | 26 | <$5M | Research |
| Villages Corral, LLC | Accommodation and Food Services | $279,930 | $282,496 | 35 | $6.2M | Research |
| Jedz, Inc. | Accommodation and Food Services | $255,153 | $257,492 | 29 | $5.1M | Research |
| Physio-Med Inc. | Health Care and Social Assistance | $253,299 | $254,777 | 25 | $6.8M | Research |
| Merrill Insurance Group, Inc. | Finance and Insurance | $250,200 | $252,805 | 26 | $13.4M | Research |