| Amelia Rental & Management Services Inc. | Real Estate and Rental and Leasing | $109,219 | $109,959 | 10 | <$5M | AI Deep Dive |
| Island Education, Inc. | Educational Services | $107,794 | $108,615 | 25 | <$5M | AI Deep Dive |
| Fernandina Investments, LLC | Accommodation and Food Services | $107,400 | $108,377 | 26 | $8.1M | AI Deep Dive |
| Cedar River Seafood of Fernandina INC | Accommodation and Food Services | $107,267 | $108,372 | 26 | <$5M | AI Deep Dive |
| Anchor Amelia Adventures LLC | Arts, Entertainment, and Recreation | $104,846 | $105,438 | 12 | <$5M | AI Deep Dive |
| Le Clos of Amelia Island INC | Accommodation and Food Services | $104,000 | $104,760 | 15 | <$5M | AI Deep Dive |
| Rrg of Amelia, Inc. | Accommodation and Food Services | $102,731 | $103,396 | 14 | <$5M | AI Deep Dive |
| Family Extended Care of Amelia Island Inc. | Health Care and Social Assistance | $102,582 | $103,656 | 17 | <$5M | AI Deep Dive |
| First Coast Pediatrics PA | Health Care and Social Assistance | $102,492 | $103,241 | 15 | <$5M | AI Deep Dive |
| Bob's Irrigation & Landscape, Inc. | Administrative and Support and Waste Management | $102,363 | $103,417 | 14 | <$5M | AI Deep Dive |
| First Coast Pediatrics, PA | Health Care and Social Assistance | $100,015 | $101,004 | 16 | $5.0M | AI Deep Dive |
| Shorebreak Inc. | Construction | $100,000 | $100,964 | 20 | $6.6M | AI Deep Dive |
| The Freezette, LLC | Accommodation and Food Services | $99,900 | $100,743 | 16 | <$5M | AI Deep Dive |
| Benjamin N. Diller, Dds, PA | Health Care and Social Assistance | $99,830 | $100,795 | 9 | <$5M | AI Deep Dive |
| Benjamin N Diller DDS PA | Health Care and Social Assistance | $99,830 | $100,975 | 10 | <$5M | AI Deep Dive |
| Richard's of Waycross Georgia INC | Accommodation and Food Services | $99,419 | $100,455 | 13 | <$5M | AI Deep Dive |
| Nassau Humane Society, Inc. | Other Services (except Public Administration) | $99,000 | $99,583 | 24 | N/A | AI Deep Dive |
| First Coast Telecom LLC | Utilities | $98,505 | $99,787 | 10 | N/A | AI Deep Dive |
| Dicks Wings | Accommodation and Food Services | $97,580 | $98,128 | 15 | <$5M | AI Deep Dive |
| Post Office Place Operations, LLC | Accommodation and Food Services | $96,900 | $97,742 | 25 | <$5M | AI Deep Dive |
| Michael A. Manzie & Associates, Inc. | Professional, Scientific, and Technical Services | $94,500 | $95,062 | 11 | <$5M | AI Deep Dive |
| Ace Painting & Maintenance Inc. | Construction | $94,200 | $94,685 | 13 | <$5M | AI Deep Dive |
| Dunlop Property Management Inc. | Accommodation and Food Services | $94,106 | $95,130 | 10 | <$5M | AI Deep Dive |
| K&m Hideaway Inc. | Retail Trade | $93,935 | $94,929 | 13 | <$5M | AI Deep Dive |
| Nassau Open Mri, LLC | Health Care and Social Assistance | $93,500 | $94,406 | 6 | <$5M | AI Deep Dive |