| Hope Hospice and Community Services INC - Had1003 | Health Care and Social Assistance | $10,000,000 | $10,043,836 | 325 | $55.7M |  Research |
| Juniper Landscaping of Florida, LLC | Administrative and Support and Waste Management | $7,636,900 | $7,721,330 | 500 | $108.3M | Research |
| Radiology Regional Center PA | Health Care and Social Assistance | $7,064,750 | $6,672,927 | 493 | $154.7M | Research |
| Family Health Centers of Southwest Florida Inc. | Health Care and Social Assistance | $5,854,482 | $5,879,038 | 500 | $89.0M | Research |
| Fallsafe Solutions, LLC | Construction | $5,769,000 | $5,847,079 | 500 | $170.4M | Research |
| Leesar, Inc. | Wholesale Trade | $5,092,135 | $5,157,060 | 476 | N/A | Research |
| Saluscare, Inc. | Health Care and Social Assistance | $3,747,500 | $3,791,117 | 369 | N/A | Research |
| Physicians' Primary Care of Southwest Florida, P.l | Health Care and Social Assistance | $3,035,393 | $3,071,228 | 252 | $44.8M | Research |
| Lee County Rv Sales Company | Retail Trade | $2,874,330 | $2,906,536 | 188 | $49.1M | Research |
| Allyn International Services INC | Professional, Scientific, and Technical Services | $2,819,227 | $2,850,045 | 239 | $24.7M | Research |
| Henderson, Franklin, Starnes & Holt, PA | Professional, Scientific, and Technical Services | $2,807,900 | $2,656,821 | 135 | $32.8M | Research |
| Cornerstone Builders of SW Florida, Inc. | Construction | $2,801,620 | $2,834,539 | 255 | $77.5M | Research |
| Sam Galloway Ford, Inc. | Retail Trade | $2,680,900 | $0 | 244 | $61.5M | Research |
| Scotlynn USA Division INC | Transportation and Warehousing | $2,593,030 | $2,387,923 | 214 | $82.9M | Research |
| D Peck Roofing INC | Construction | $2,560,807 | $416,877 | 36 | $11.9M | Research |
| Clive Daniel Home Holdings LLC | Wholesale Trade | $2,553,200 | $2,583,279 | 185 | $56.7M | Research |
| Ad-Ler Roofing Inc. | Construction | $2,504,100 | $2,526,122 | 194 | $64.0M | Research |
| O'brien Automotive of Florida LLC | Retail Trade | $2,439,375 | $2,466,977 | 181 | $45.6M | Research |
| Jof LLC | Accommodation and Food Services | $2,294,875 | $2,320,820 | 500 | $88.8M | Research |
| Robb & Stucky International, Inc. | Retail Trade | $2,227,770 | $2,253,761 | 169 | $30.6M | Research |
| Eye Centers of Florida PA | Health Care and Social Assistance | $2,146,913 | $2,171,499 | 190 | $44.5M | Research |
| Fort Myers Broadcasting Company | Information | $2,122,132 | $2,146,086 | 223 | $37.4M | Research |
| Perfusion.com, INC | Health Care and Social Assistance | $2,120,000 | $2,144,685 | 93 | $17.0M | Research |
| Youngquist Brothers Oil & Gas, N.d., INC | Mining, Quarrying, and Oil and Gas Extraction | $2,047,392 | $0 | 61 | <$5M | Research |
| Crowther Holdings, Inc. | Construction | $2,040,000 | $2,064,027 | 175 | $57.7M | Research |