| Simedhealth LLC | Health Care and Social Assistance | $6,123,402 | $6,195,352 | 474 | $148.8M |  Research |
| Info Tech, INC | Other Services (except Public Administration) | $5,569,147 | $5,657,016 | 303 | $57.9M | Research |
| Meridian Behavioral Healthcare, Inc. | Health Care and Social Assistance | $5,403,600 | $5,463,940 | 500 | N/A | Research |
| Paradigm Management Team, LLC | Real Estate and Rental and Leasing | $4,654,531 | $4,707,580 | 419 | $62.9M | Research |
| Infinite Energy Inc. | Utilities | $4,431,500 | $4,481,601 | 309 | N/A | Research |
| Charles Perry Partners, Inc. | Construction | $3,504,642 | $3,545,822 | 219 | $66.5M | Research |
| Double 18, Inc. | Accommodation and Food Services | $3,467,100 | $286,935 | 71 | $12.6M | Research |
| Sharpspring Technologies, Inc. | Professional, Scientific, and Technical Services | $3,234,000 | $3,271,101 | 227 | $31.8M | Research |
| Environmental Consulting & Technology, Inc. | Professional, Scientific, and Technical Services | $3,078,200 | $3,400,221 | 182 | $18.8M | Research |
| O2b Early Education Holding, Inc. | Health Care and Social Assistance | $2,686,200 | $2,349,215 | 499 | $80.8M | Research |
| James Moore & Co., P.l. | Professional, Scientific, and Technical Services | $2,424,500 | $2,452,398 | 177 | $56.3M | Research |
| Interventional Cardiologists of Gainesville, PA | Health Care and Social Assistance | $2,400,000 | $2,432,600 | 170 | $53.4M | Research |
| Ray's Metal Works, INC | Construction | $2,387,673 | $2,414,689 | 140 | $44.7M | Research |
| Oak Hammock at the University of Florida, Inc. | Health Care and Social Assistance | $2,173,100 | $2,197,970 | 337 | $57.3M | Research |
| Jones Edmunds & Associates, Inc. | Other Services (except Public Administration) | $2,000,000 | $0 | 149 | $28.5M | Research |
| Comfort Temp Company | Manufacturing | $1,947,225 | $1,967,238 | 168 | $44.0M | Research |
| Innovative Scheduling LLC | Professional, Scientific, and Technical Services | $1,818,203 | $1,610,988 | 118 | $24.4M | Research |
| Innovative Scheduling, LLC | Professional, Scientific, and Technical Services | $1,818,200 | $1,837,796 | 52 | $5.4M | Research |
| Purvis Gray and Company LLP | Professional, Scientific, and Technical Services | $1,702,594 | $1,709,073 | 106 | $33.7M | Research |
| Comfort Temp Company | Construction | $1,683,050 | $1,697,122 | 175 | $55.4M | Research |
| Archer Health Ventures LLC | Health Care and Social Assistance | $1,644,700 | $1,662,949 | 224 | $36.3M | Research |
| Purvis, Gray and Company, LLP | Professional, Scientific, and Technical Services | $1,556,064 | $1,568,080 | 114 | $36.3M | Research |
| Shadow Health, Inc. | Professional, Scientific, and Technical Services | $1,534,500 | $1,542,258 | 113 | $23.4M | Research |
| The Arc of Alachua County, Inc. | Health Care and Social Assistance | $1,511,300 | $1,526,665 | 215 | N/A | Research |
| Gleim Publications INC | Information | $1,498,600 | $1,507,797 | 128 | $21.5M | Research |