| Vac-Con, Inc. | Manufacturing | $4,255,507 | $4,312,720 | 326 | $85.4M |  Research |
| Vallencourt Construction Co., Inc. | Construction | $4,050,260 | $4,097,310 | 388 | $89.7M | Research |
| Mabro Marine, Inc. | Transportation and Warehousing | $1,916,182 | $1,934,865 | 120 | $9.3M | Research |
| Mean Greene Logistics LLC | Transportation and Warehousing | $1,516,925 | $1,537,783 | 77 | $17.6M | Research |
| High Tide Partners LLC | Unknown | $1,298,570 | $0 | 166 | N/A | Research |
| Challenge Enterprises of North Florida, Inc. | Administrative and Support and Waste Management | $1,125,900 | $1,134,339 | 320 | N/A | Research |
| Garber Chevrolet Buick Gmc INC | Retail Trade | $1,029,577 | $1,040,916 | 91 | $22.9M | Research |
| Hard Rock Materials, INC | Construction | $820,600 | $828,087 | 71 | $16.4M | Research |
| Smith Maritime INC | Transportation and Warehousing | $650,480 | $659,248 | 17 | <$5M | Research |
| Bartram Trail Surveying, Inc. | Professional, Scientific, and Technical Services | $637,417 | $641,755 | 71 | $14.3M | Research |
| Jaquar Concrete INC | Unknown | $511,117 | $519,029 | 57 | N/A | Research |
| Genplant On-Site Energy LLC | Real Estate and Rental and Leasing | $507,533 | $514,469 | 32 | <$5M | Research |
| Walker Footings, Inc. | Construction | $481,572 | $487,733 | 40 | $9.3M | Research |
| Smith Maritime INC | Transportation and Warehousing | $472,500 | $655,470 | 35 | $8.9M | Research |
| D & M Trim INC | Construction | $471,894 | $477,427 | 37 | $11.2M | Research |
| Trinity Fabricators INC | Manufacturing | $468,055 | $472,210 | 38 | $9.3M | Research |
| Trinity Fabricators Inc. | Manufacturing | $468,055 | $470,479 | 37 | $9.0M | Research |
| Garber Chrysler Dodge Truck INC | Retail Trade | $445,967 | $450,879 | 42 | $10.6M | Research |
| Genplant On Site Energy LLC | Professional, Scientific, and Technical Services | $426,407 | $429,641 | 29 | <$5M | Research |
| George B Wittmer Associates INC | Administrative and Support and Waste Management | $393,780 | $398,948 | 34 | $6.4M | Research |
| George B Wittmer Associates INC | Construction | $393,780 | $397,869 | 26 | $8.9M | Research |
| Southern Scapes INC | Administrative and Support and Waste Management | $376,300 | $378,950 | 26 | $5.6M | Research |
| Garber Ford INC | Retail Trade | $341,157 | $344,942 | 34 | $8.6M | Research |
| Homeactions LLC | Professional, Scientific, and Technical Services | $284,467 | $287,675 | 26 | $5.4M | Research |
| Executive Food Services INC | Accommodation and Food Services | $281,816 | $284,426 | 25 | <$5M | Research |