| Collier Health Services, INC | Health Care and Social Assistance | $4,875,350 | $4,930,247 | 331 | N/A |  Research |
| Oakes Farms, INC | Agriculture, Forestry, Fishing and Hunting | $3,229,325 | $3,297,539 | 355 | $36.3M | Research |
| Ave Marie University. INC | Educational Services | $2,700,197 | $2,731,474 | 266 | N/A | Research |
| Oakes Farm Op LLC | Agriculture, Forestry, Fishing and Hunting | $1,486,003 | $1,508,557 | 270 | $27.6M | Research |
| Oakes Farm Op, LLC | Accommodation and Food Services | $1,486,003 | $1,517,310 | 270 | $50.4M | Research |
| Lesman Ayala | Other Services (except Public Administration) | $800,700 | $809,107 | 266 | $50.8M | Research |
| Oakes Farms Market, LLC | Retail Trade | $705,035 | $719,870 | 146 | $16.2M | Research |
| Primate Products LLC | Agriculture, Forestry, Fishing and Hunting | $597,700 | $605,038 | 40 | $6.5M | Research |
| Hbs Florida Specialties LLC | Agriculture, Forestry, Fishing and Hunting | $412,200 | $415,967 | 91 | <$5M | Research |
| S & O Grocers INC | Retail Trade | $366,300 | $305,052 | 62 | $15.5M | Research |
| Fletcher Flying Services INC | Agriculture, Forestry, Fishing and Hunting | $340,898 | $345,585 | 16 | <$5M | Research |
| Alliance Packing LLC | Agriculture, Forestry, Fishing and Hunting | $315,543 | $316,995 | 30 | <$5M | Research |
| Saint John Children Village INC | Other Services (except Public Administration) | $312,625 | $0 | 15 | N/A | Research |
| Sandoval Wholesales, Inc. | Wholesale Trade | $312,500 | $334,424 | 84 | $25.7M | Research |
| Liquid Plant INC | Wholesale Trade | $269,100 | $270,745 | 21 | $5.4M | Research |
| Barnett Partin Plants INC | Agriculture, Forestry, Fishing and Hunting | $252,600 | $254,123 | 31 | <$5M | Research |
| Southern Corporate Packers INC | Wholesale Trade | $240,000 | $242,992 | 45 | $11.6M | Research |
| Leal Harvesting & Hauling, Inc. | Agriculture, Forestry, Fishing and Hunting | $232,900 | $235,307 | 150 | $7.5M | Research |
| Empire Tile of Naples INC | Construction | $225,436 | $227,400 | 25 | $6.3M | Research |
| Coalition of Immokalee Workers Inc, the | Other Services (except Public Administration) | $224,282 | $226,009 | 20 | N/A | Research |
| Coalition of Immokalee Workers | Other Services (except Public Administration) | $201,205 | $202,285 | 19 | N/A | Research |
| Blocker & Lewis Enterprises Inc. | Retail Trade | $186,500 | $187,940 | 28 | $5.8M | Research |
| Salazar Machine and Steel Inc. | Manufacturing | $168,203 | $169,825 | 13 | <$5M | Research |
| Salazar Machine and Steel INC | Manufacturing | $168,203 | $169,138 | 14 | <$5M | Research |
| Lozano's Restaurant & Sports Barinc | Accommodation and Food Services | $168,000 | $169,633 | 24 | <$5M | Research |