| Johnson & Johnson INC | Retail Trade | $2,255,700 | $2,281,390 | 250 | $83.7M |  Research |
| Solaris Healthcare Lake City, LLC | Health Care and Social Assistance | $1,247,195 | $1,262,127 | 186 | $31.6M | Research |
| J-Quint, LLC | Accommodation and Food Services | $1,162,800 | $1,174,842 | 500 | $88.8M | Research |
| Lake City F Automotive Management, LLC | Other Services (except Public Administration) | $1,051,900 | $1,063,687 | 89 | $17.0M | Research |
| Cdt USA INC | Transportation and Warehousing | $1,050,445 | $1,060,658 | 102 | $20.6M | Research |
| Great South Timber & Lumber LLC | Manufacturing | $975,600 | $775,991 | 60 | $59.5M | Research |
| Lake City Mgt LLC | Health Care and Social Assistance | $926,422 | $936,904 | 105 | $17.9M | Research |
| Frenvey, INC | Retail Trade | $919,147 | $928,976 | 209 | $52.2M | Research |
| Quality Industries of America INC | Manufacturing | $819,825 | $823,651 | 74 | $19.4M | Research |
| Quality Industries of America, Inc. | Manufacturing | $803,990 | $808,836 | 68 | $16.6M | Research |
| North Florida Pediatric PA | Health Care and Social Assistance | $795,520 | $802,127 | 95 | $29.8M | Research |
| Townhomes, LLC | Manufacturing | $649,200 | $652,717 | 105 | $104.1M | Research |
| Townhomes LLC | Manufacturing | $649,100 | $652,544 | 105 | $104.1M | Research |
| Cal-Tech Testing, Inc. | Construction | $615,300 | $618,889 | 108 | $25.0M | Research |
| Lake City T Automotive Management, LLC | Retail Trade | $573,100 | $578,580 | 52 | $13.1M | Research |
| Lake City J Automotive Management LLC | Retail Trade | $563,000 | $567,350 | 48 | $12.1M | Research |
| Lake City G Automotive Management, LLC | Retail Trade | $528,700 | $492,923 | 53 | $13.4M | Research |
| Andres R Villar MD PA | Health Care and Social Assistance | $468,500 | $471,220 | 33 | $6.5M | Research |
| Columbia Grain & Ingredients INC | Transportation and Warehousing | $455,640 | $460,855 | 44 | $11.7M | Research |
| Columbia Eye Associates PA | Health Care and Social Assistance | $454,000 | $456,611 | 51 | $16.0M | Research |
| Rdh Trucking INC | Transportation and Warehousing | $439,125 | $441,019 | 39 | $7.9M | Research |
| G&s Nursery LLC | Retail Trade | $435,447 | $438,689 | 44 | $9.1M | Research |
| Lotus Logic, Inc. | Professional, Scientific, and Technical Services | $432,460 | $438,346 | 26 | $5.4M | Research |
| G&s Nursery LLC | Retail Trade | $427,707 | $430,962 | 42 | $8.7M | Research |
| North Florida Pharmacy, Inc. | Retail Trade | $420,700 | $423,797 | 53 | $9.2M | Research |