| Millers Central Air INC | Construction | $613,100 | $618,022 | 61 | $19.3M |  Research |
| Lake Placid Caretakers INC | Agriculture, Forestry, Fishing and Hunting | $455,977 | $458,536 | 39 | <$5M | Research |
| Sb Harvesting INC | Agriculture, Forestry, Fishing and Hunting | $428,622 | $0 | 40 | <$5M | Research |
| Heartland Metals, Inc. | Manufacturing | $398,600 | $401,171 | 43 | $10.5M | Research |
| Smoak Groves INC | Agriculture, Forestry, Fishing and Hunting | $377,900 | $379,737 | 41 | <$5M | Research |
| Family Medical Group, P.a. | Health Care and Social Assistance | $302,727 | $305,182 | 28 | $8.8M | Research |
| Ls Barajas INC | Agriculture, Forestry, Fishing and Hunting | $272,668 | $276,373 | 90 | <$5M | Research |
| Jbo Harvesting, INC | Agriculture, Forestry, Fishing and Hunting | $272,300 | $275,341 | 91 | $14.9M | Research |
| Family Medical Group P.a. | Health Care and Social Assistance | $266,817 | $268,522 | 33 | $10.4M | Research |
| Central Florida Yamaha INC | Retail Trade | $261,974 | $264,688 | 22 | $5.7M | Research |
| Wheeler Farms, Inc. | Agriculture, Forestry, Fishing and Hunting | $248,035 | $250,288 | 29 | <$5M | Research |
| Lake Placid Camp & Conference Center | Accommodation and Food Services | $231,298 | $233,427 | 40 | N/A | Research |
| Central Contractors of Highlands County INC | Construction | $231,122 | $232,002 | 18 | $6.1M | Research |
| Central Contractors of Highlands County, INC | Construction | $222,582 | $224,684 | 20 | $6.8M | Research |
| Optimum Agriculture Fl LLC | Agriculture, Forestry, Fishing and Hunting | $206,700 | $209,441 | 43 | <$5M | Research |
| Bevis Construction, Inc. | Construction | $203,555 | $204,935 | 20 | $6.1M | Research |
| Florida Grove Hedgers Inc. | Agriculture, Forestry, Fishing and Hunting | $202,407 | $204,298 | 21 | <$5M | Research |
| Bevis Construction Inc. | Construction | $202,022 | $203,004 | 22 | $6.7M | Research |
| Florida Grove Hedgers, INC | Agriculture, Forestry, Fishing and Hunting | $199,500 | $201,227 | 21 | <$5M | Research |
| Griffin Trees, Inc. | Agriculture, Forestry, Fishing and Hunting | $195,557 | $196,622 | 21 | <$5M | Research |
| C & C Pumbing and Repair, Inc. | Construction | $190,242 | $191,342 | 14 | <$5M | Research |
| G C L P Inc. | Accommodation and Food Services | $163,502 | $165,160 | 30 | $5.3M | Research |
| Scott Gordon | Transportation and Warehousing | $155,060 | $155,914 | 10 | <$5M | Research |
| Lake Placid Camp and Conference Center, Inc. | Accommodation and Food Services | $153,100 | $155,151 | 40 | N/A | Research |
| Heartland Marble, Inc. | Construction | $152,600 | $153,595 | 24 | $8.2M | Research |