| Sanlando United Methodist Church INC | Other Services (except Public Administration) | $173,821 | $174,768 | 43 | N/A | AI Deep Dive |
| Cg2 Holdings, INC | Health Care and Social Assistance | $173,510 | $174,389 | 61 | $10.4M | AI Deep Dive |
| Papa Bees, INC | Accommodation and Food Services | $173,300 | $175,283 | 29 | $5.1M | AI Deep Dive |
| Yuk Ming Inc. | Accommodation and Food Services | $172,262 | $174,061 | 200 | $35.5M | AI Deep Dive |
| Orlando Diagnostic Center | Health Care and Social Assistance | $170,700 | $172,870 | 17 | <$5M | AI Deep Dive |
| The Hourglass Brewery, LLC | Manufacturing | $168,800 | $170,826 | 26 | $9.2M | AI Deep Dive |
| 5ifty & 5ive LLC | Professional, Scientific, and Technical Services | $168,447 | $170,657 | 13 | <$5M | AI Deep Dive |
| Bono and Associates, LLC | Real Estate and Rental and Leasing | $168,200 | $170,204 | 29 | $5.3M | AI Deep Dive |
| The Coordinator, Inc. | Accommodation and Food Services | $167,820 | $168,767 | 9 | <$5M | AI Deep Dive |
| Seminole Restaurants of Florida, Inc. | Accommodation and Food Services | $167,710 | $168,819 | 36 | $6.4M | AI Deep Dive |
| Titan Mechanical INC | Construction | $167,434 | $168,057 | 13 | <$5M | AI Deep Dive |
| Ao Shun Inc. | Accommodation and Food Services | $167,100 | $169,374 | 26 | <$5M | AI Deep Dive |
| Stages of Life Medical Institute INC | Health Care and Social Assistance | $165,856 | $167,583 | 17 | $5.3M | AI Deep Dive |
| North Orlando Spine Center, LLC | Health Care and Social Assistance | $165,500 | $166,427 | 20 | <$5M | AI Deep Dive |
| Handy Air and Solar LLC | Construction | $165,200 | $89,259 | 24 | $8.2M | AI Deep Dive |
| First Baptist Church of Longwood | Other Services (except Public Administration) | $164,500 | $166,298 | 33 | N/A | AI Deep Dive |
| R K Edwards INC | Finance and Insurance | $164,394 | $166,348 | 16 | $8.2M | AI Deep Dive |
| Zeppe's Pizzeria of Hudson, Inc. | Accommodation and Food Services | $162,120 | $163,675 | 57 | $10.1M | AI Deep Dive |
| J and M Roofing Services INC | Construction | $161,606 | $163,425 | 18 | $5.9M | AI Deep Dive |
| A Sun State Trees, Inc. | Administrative and Support and Waste Management | $161,300 | $163,478 | 35 | $7.6M | AI Deep Dive |
| Progressive Distribution, LLC | Wholesale Trade | $160,451 | $161,686 | 24 | $6.2M | AI Deep Dive |
| Finishing Systems of Florida, Inc. | Administrative and Support and Waste Management | $159,592 | $161,813 | 15 | <$5M | AI Deep Dive |
| Longwood Lb, LLC | Health Care and Social Assistance | $158,822 | $160,075 | 35 | $5.7M | AI Deep Dive |
| Longwood Lb LLC | Health Care and Social Assistance | $158,822 | $159,748 | 35 | $5.7M | AI Deep Dive |
| St. Stephen Lutheran Church of South Seminole | Other Services (except Public Administration) | $157,800 | $158,804 | 30 | N/A | AI Deep Dive |