| Baker Medical Services INC | Health Care and Social Assistance | $3,555,800 | $3,595,255 | 375 | $74.4M |  Research |
| Burnham Construction, Inc. | Construction | $907,900 | $912,919 | 65 | $20.1M | Research |
| Earthworks of Florida LLC | Construction | $805,300 | $809,845 | 55 | $17.0M | Research |
| Earthworks of Florida LLC | Construction | $801,260 | $806,177 | 75 | $23.2M | Research |
| Dr Roof INC | Construction | $369,000 | $371,112 | 42 | $13.8M | Research |
| Burkins Chevrolet, LLC | Retail Trade | $328,000 | $330,399 | 52 | $13.1M | Research |
| Rgms, Inc. | Accommodation and Food Services | $294,300 | $296,171 | 75 | $13.3M | Research |
| Davis & Davis Enterprises INC | Retail Trade | $276,664 | $278,627 | 58 | $19.4M | Research |
| Davis & Davis Enterprises INC | Retail Trade | $238,700 | $240,459 | 38 | $12.7M | Research |
| Bradley Surveying & Mapping, INC | Professional, Scientific, and Technical Services | $237,700 | $239,149 | 26 | $5.2M | Research |
| Alachua Enviromental Services, Inc. | Administrative and Support and Waste Management | $224,690 | $226,284 | 38 | $7.2M | Research |
| Crews Electrical Testing INC | Professional, Scientific, and Technical Services | $204,900 | $207,106 | 11 | <$5M | Research |
| Rentavision LLC | Retail Trade | $182,500 | $76,785 | 21 | $5.2M | Research |
| Michal Harris Holdings, PA | Professional, Scientific, and Technical Services | $178,000 | $178,984 | 22 | <$5M | Research |
| Margaret L Romeo Dmd PA | Health Care and Social Assistance | $144,400 | $145,298 | 19 | <$5M | Research |
| Macclenny Bbq LLC | Accommodation and Food Services | $137,500 | $125,832 | 42 | $7.5M | Research |
| Lyons and Lyons Cpa's | Professional, Scientific, and Technical Services | $133,700 | $134,524 | 9 | <$5M | Research |
| Stone Transport Services LLC | Transportation and Warehousing | $132,300 | $133,425 | 13 | <$5M | Research |
| M&r Specialty Trailers & Trucks INC | Manufacturing | $127,500 | $128,684 | 16 | <$5M | Research |
| Dependable Heating, Air Conditioning & Electrical Contractor | Construction | $123,600 | $124,359 | 14 | <$5M | Research |
| Wayne Frier Home Center of Macclenny, LLC | Retail Trade | $121,700 | $122,528 | 11 | <$5M | Research |
| M&r Specialty Trailers & Trucks INC | Manufacturing | $118,200 | $119,172 | 11 | <$5M | Research |
| Miracle Automotive & Truck Service Center INC | Transportation and Warehousing | $110,200 | $110,889 | 10 | <$5M | Research |
| Diversified Management Group LLC | Administrative and Support and Waste Management | $109,580 | $110,406 | 29 | $6.3M | Research |
| Crystal River Seafood of Macclenny, Inc. | Accommodation and Food Services | $104,000 | $104,704 | 21 | <$5M | Research |