| Mayo Fertilizer | Wholesale Trade | $1,076,200 | $1,086,872 | 113 | $29.1M | AI Deep Dive |
| Lafayette Operations LLC | Health Care and Social Assistance | $458,800 | $464,968 | 64 | $10.9M | AI Deep Dive |
| Triple P Trucking, INC | Transportation and Warehousing | $314,100 | $315,758 | 22 | <$5M | AI Deep Dive |
| Putnals Premium Pine Straw, Inc. | Agriculture, Forestry, Fishing and Hunting | $234,000 | $235,235 | 13 | <$5M | AI Deep Dive |
| White Oak Dairy, Inc. | Agriculture, Forestry, Fishing and Hunting | $176,000 | $176,958 | 24 | <$5M | AI Deep Dive |
| Bass Assassin Lures, INC | Manufacturing | $141,800 | $121,071 | 19 | $5.3M | AI Deep Dive |
| Alberto Olguin | Wholesale Trade | $126,700 | $127,330 | 1 | <$5M | AI Deep Dive |
| Juan Ceron PA | Real Estate and Rental and Leasing | $121,242 | $122,109 | 10 | <$5M | AI Deep Dive |
| Triple L Cattle Company LLC | Agriculture, Forestry, Fishing and Hunting | $120,612 | $121,721 | 44 | $7.2M | AI Deep Dive |
| Triple L Cattle Company, LLC | Agriculture, Forestry, Fishing and Hunting | $120,600 | $122,278 | 44 | $7.2M | AI Deep Dive |
| Mayo Sales, INC | Retail Trade | $119,600 | $120,464 | 22 | $5.5M | AI Deep Dive |
| Pearsons Ready Mix Concrete INC | Manufacturing | $115,500 | $116,446 | 14 | $13.9M | AI Deep Dive |
| Mayo Truss Company, INC | Manufacturing | $104,548 | $105,287 | 17 | $16.9M | AI Deep Dive |
| Singletary Trucking & Hauling Company | Transportation and Warehousing | $83,240 | $83,892 | 12 | <$5M | AI Deep Dive |
| Pine House, INC | Health Care and Social Assistance | $82,097 | $82,690 | 25 | <$5M | AI Deep Dive |
| Mcadams Dairy Farm INC | Agriculture, Forestry, Fishing and Hunting | $80,987 | $81,471 | 13 | <$5M | AI Deep Dive |
| Mcadams Dairy Farm, INC | Agriculture, Forestry, Fishing and Hunting | $80,900 | $81,637 | 13 | <$5M | AI Deep Dive |
| W. R. Williams Distributor, INC | Retail Trade | $76,100 | $76,776 | 12 | <$5M | AI Deep Dive |
| J & J Gas Service, INC | Retail Trade | $72,800 | $73,631 | 9 | <$5M | AI Deep Dive |
| North Florida Pharmacy of Mayo Inc. | Retail Trade | $70,778 | $59,969 | 8 | <$5M | AI Deep Dive |
| Sorensen Smith & Bay LLC | Health Care and Social Assistance | $63,315 | $63,844 | 16 | <$5M | AI Deep Dive |
| Lighthouse Christian Center of Mayo, Inc. | Other Services (except Public Administration) | $60,000 | $60,480 | 16 | N/A | AI Deep Dive |
| Organic Growing Solutions LLC | Manufacturing | $56,500 | $56,775 | 6 | $6.0M | AI Deep Dive |
| Cent Fl Lands & Timber Nursey | Agriculture, Forestry, Fishing and Hunting | $56,100 | $56,561 | 14 | <$5M | AI Deep Dive |
| Organic Growing Solutions, LLC | Manufacturing | $55,696 | $56,138 | 6 | $6.0M | AI Deep Dive |