| Tropic Care of Florida INC | Administrative and Support and Waste Management | $10,000,000 | $342,204 | 50 | $10.8M |  Research |
| Brph Architects Engineers INC | Professional, Scientific, and Technical Services | $6,185,400 | $6,258,766 | 328 | $101.7M | Research |
| Revolution Technologies, LLC | Professional, Scientific, and Technical Services | $5,606,500 | $5,670,245 | 347 | $35.9M | Research |
| Sun Nuclear Corp | Manufacturing | $5,244,598 | $5,302,073 | 250 | $65.5M | Research |
| Italian Terrazo and Tile Co. of Brevard | Wholesale Trade | $4,880,091 | $4,942,718 | 402 | $123.1M | Research |
| Circles of Care, Inc. | Health Care and Social Assistance | $4,801,165 | $4,856,245 | 457 | N/A | Research |
| Brevard Physician Associates Pllc | Health Care and Social Assistance | $4,793,700 | $4,849,627 | 292 | $91.7M | Research |
| The Brevard Health Alliance, Inc. | Health Care and Social Assistance | $4,693,495 | $4,728,566 | 336 | N/A | Research |
| Coastal Mechanical Services, LLC | Construction | $4,474,858 | $2,915,233 | 312 | $99.5M | Research |
| Medical Associates of Brevard LLC | Health Care and Social Assistance | $3,208,952 | $3,251,827 | 265 | $83.2M | Research |
| Holiday Builders INC | Construction | $2,847,900 | $0 | 164 | $49.8M | Research |
| Medicomp INC | Health Care and Social Assistance | $2,004,479 | $2,027,607 | 160 | $37.1M | Research |
| Brevard Physician Associates Pllc | Health Care and Social Assistance | $2,000,000 | $2,019,278 | 221 | $69.4M | Research |
| Medicomp INC | Manufacturing | $1,995,183 | $2,013,361 | 189 | $49.5M | Research |
| Calvary Chapel Fellowship of Melbourne | Other Services (except Public Administration) | $1,983,500 | $2,009,341 | 324 | N/A | Research |
| Avidyne Corporation | Manufacturing | $1,954,645 | $1,973,486 | 118 | $30.9M | Research |
| Southeast Aerospace, Inc. | Wholesale Trade | $1,907,745 | $1,931,115 | 115 | $35.2M | Research |
| Carroll Distributing Company | Wholesale Trade | $1,867,604 | $1,882,449 | 135 | $34.7M | Research |
| Avidyne Corporation | Manufacturing | $1,847,067 | $1,856,353 | 150 | $39.3M | Research |
| Patronus Systems INC | Administrative and Support and Waste Management | $1,828,713 | $1,845,046 | 218 | $31.9M | Research |
| Indian River Center LLC DBA Indian River Center | Health Care and Social Assistance | $1,707,495 | $1,721,914 | 164 | $27.9M | Research |
| Holy Trinity Episcopal Academy, Inc. | Educational Services | $1,612,200 | $1,623,198 | 147 | N/A | Research |
| Deland Senior Care LLC | Health Care and Social Assistance | $1,592,173 | $1,602,345 | 200 | $34.0M | Research |
| Boys Electrical Contractors LLC | Construction | $1,486,800 | $1,137,103 | 150 | $49.4M | Research |
| Omni Healthcare INC | Health Care and Social Assistance | $1,471,765 | $1,491,634 | 153 | $35.5M | Research |