| Florida Center for Orthopaedics | Health Care and Social Assistance | $300,000 | $302,063 | 21 | $6.6M |  Research |
| Masney Auto Transport, INC | Transportation and Warehousing | $286,700 | $289,153 | 20 | <$5M | Research |
| Sagacious Stone, LLC | Construction | $279,777 | $283,464 | 12 | <$5M | Research |
| Keator Construction LLC | Construction | $279,000 | $280,139 | 24 | $27.3M | Research |
| Keator Construction, LLC | Construction | $279,000 | $280,521 | 24 | $27.3M | Research |
| Ocoee Glad Tidings Assembly of | Other Services (except Public Administration) | $277,600 | $279,768 | 89 | N/A | Research |
| Territo Corporation | Information | $267,272 | $268,912 | 16 | <$5M | Research |
| Territo Corporation | Professional, Scientific, and Technical Services | $267,272 | $269,293 | 16 | <$5M | Research |
| Superior Roadway Services LLC | Construction | $267,200 | $268,928 | 31 | $9.6M | Research |
| Fpis INC | Professional, Scientific, and Technical Services | $264,662 | $0 | 32 | <$5M | Research |
| Orlando Wine Company, LLC | Accommodation and Food Services | $264,100 | $265,458 | 52 | $9.2M | Research |
| Fpis Inc. | Professional, Scientific, and Technical Services | $248,607 | $0 | 36 | <$5M | Research |
| Neurosurgical Consultants of Florida, PA | Health Care and Social Assistance | $244,782 | $246,599 | 15 | <$5M | Research |
| American Sanitary Partition Corp | Manufacturing | $234,777 | $237,491 | 26 | $9.2M | Research |
| Cim-Tech.com, Inc. | Professional, Scientific, and Technical Services | $228,594 | $230,354 | 12 | <$5M | Research |
| Neurosurgical Consultants of Florida, PA | Health Care and Social Assistance | $225,000 | $227,799 | 15 | <$5M | Research |
| Mirpuri Creations Inc. | Retail Trade | $218,065 | $219,899 | 24 | $6.6M | Research |
| Acy Contractors Llc. | Construction | $216,096 | $0 | 20 | $6.8M | Research |
| Mirpuri Creations, Inc. | Retail Trade | $214,000 | $215,735 | 36 | $10.0M | Research |
| Adam Willson LLC | Transportation and Warehousing | $208,750 | $212,605 | 17 | <$5M | Research |
| H & S Electrical, Inc. | Construction | $208,333 | $211,174 | 22 | $7.2M | Research |
| Gator's Dockside of Ocoee INC | Accommodation and Food Services | $207,844 | $209,749 | 68 | $12.1M | Research |
| Quality Vaults INC | Other Services (except Public Administration) | $207,400 | $211,024 | 26 | <$5M | Research |
| Crm Learning L.l.c. | Health Care and Social Assistance | $206,908 | $208,399 | 37 | $6.0M | Research |
| 1st Choice Door & Millwork, Inc. | Construction | $203,700 | $204,950 | 22 | $6.9M | Research |