| Mid-Florida Hematology and Oncology Centers PA | Health Care and Social Assistance | $2,548,000 | $1,394,007 | 111 | $34.8M |  Research |
| First Quality Plumbing and Irrigation, INC | Construction | $2,017,100 | $2,040,577 | 160 | $50.6M | Research |
| Superior Sheds INC | Manufacturing | $1,161,192 | $1,172,136 | 116 | $115.1M | Research |
| Chinchor Electric INC | Construction | $993,000 | $998,927 | 110 | $36.2M | Research |
| All Terrain Tractor Service, Inc. | Construction | $825,800 | $836,581 | 86 | $32.2M | Research |
| Family Matters LLC | Accommodation and Food Services | $640,100 | $646,238 | 214 | $38.0M | Research |
| Professional Sunshine Roofing INC | Construction | $623,900 | $628,961 | 77 | $25.4M | Research |
| Harty Tractor Services INC | Construction | $613,900 | $620,704 | 52 | $19.5M | Research |
| Professional Sunshine Roofing INC | Construction | $575,924 | $579,444 | 50 | $16.5M | Research |
| Gel LLC | Wholesale Trade | $530,343 | $534,469 | 78 | $23.9M | Research |
| Howard J. Sakowitz, Md, PA | Health Care and Social Assistance | $462,500 | $467,857 | 50 | $15.7M | Research |
| Howard J. Sakowitz MD PA | Health Care and Social Assistance | $462,455 | $465,140 | 39 | $12.2M | Research |
| Atlantic Ear Nose & Throat PA | Health Care and Social Assistance | $455,900 | $458,572 | 40 | $12.6M | Research |
| Metrix LLC | Construction | $402,143 | $406,210 | 47 | $15.0M | Research |
| Metrix LLC | Construction | $382,700 | $387,016 | 17 | $5.4M | Research |
| Mechanical Insulation & Technologies, LLC | Construction | $359,512 | $361,689 | 40 | $13.6M | Research |
| Central Florida Medical Associates | Health Care and Social Assistance | $325,535 | $0 | 31 | $9.7M | Research |
| Pulmonary Practice Assoc | Health Care and Social Assistance | $316,293 | $318,727 | 35 | $11.0M | Research |
| Orange City Surgical, LLC | Health Care and Social Assistance | $311,200 | $313,327 | 34 | $10.7M | Research |
| Nine Square Roofing and Construction, LLC | Construction | $309,290 | $311,111 | 25 | $8.2M | Research |
| First Baptist Church Holding Company of Orange City Florida, | Other Services (except Public Administration) | $296,480 | $299,955 | 28 | N/A | Research |
| Cjr Concrete INC | Construction | $283,033 | $285,980 | 20 | $22.8M | Research |
| Cjr Concrete INC | Construction | $283,032 | $284,614 | 22 | $5.1M | Research |
| Sandcastle Care Ii, LLC | Health Care and Social Assistance | $280,100 | $282,264 | 178 | $30.5M | Research |
| Jm Spray Inc. | Construction | $267,510 | $269,412 | 42 | $10.6M | Research |