| Gray Robinson PA | Professional, Scientific, and Technical Services | $10,000,000 | $10,120,822 | 500 | $121.5M |  Research |
| Bertuccis Restaurants LLC | Accommodation and Food Services | $10,000,000 | $10,126,667 | 500 | $88.8M | Research |
| Grand Performer Inc. | Administrative and Support and Waste Management | $10,000,000 | $10,134,521 | 500 | $78.7M | Research |
| Bravo Brio Restaurants LLC | Accommodation and Food Services | $10,000,000 | $10,098,630 | 500 | $88.8M | Research |
| Buca Restaurants 2 INC | Accommodation and Food Services | $10,000,000 | $10,128,889 | 500 | $88.8M | Research |
| Red Lobster Restaurants LLC | Accommodation and Food Services | $10,000,000 | $10,064,932 | 467 | $82.9M | Research |
| Aspire Health Partners, Inc. | Health Care and Social Assistance | $10,000,000 | $10,120,548 | 500 | N/A | Research |
| Luminar Technologies, Inc. | Professional, Scientific, and Technical Services | $7,827,902 | $0 | 341 | $70.5M | Research |
| Planet Hollywood International INC | Accommodation and Food Services | $7,274,800 | $1,754,181 | 500 | $88.8M | Research |
| Buca Restaurants INC | Accommodation and Food Services | $7,259,200 | $7,352,561 | 500 | $88.8M | Research |
| Tavistock Freebirds, LLC | Accommodation and Food Services | $7,134,916 | $7,228,263 | 500 | $88.8M | Research |
| Regal Marine Industries INC | Manufacturing | $7,080,000 | $7,162,403 | 500 | $130.9M | Research |
| Mears Destination Services Inc. | Transportation and Warehousing | $7,011,637 | $7,089,131 | 412 | $158.4M | Research |
| Entertainment Technology Partners LLC | Real Estate and Rental and Leasing | $6,822,300 | $6,903,978 | 492 | $222.3M | Research |
| Drmp, Inc. | Professional, Scientific, and Technical Services | $6,644,148 | $6,724,424 | 488 | $60.2M | Research |
| Foundry Commercial, LLC | Real Estate and Rental and Leasing | $6,240,367 | $5,964,219 | 350 | $40.8M | Research |
| Assistrx Inc. | Information | $6,067,800 | $7,501,475 | 498 | $83.6M | Research |
| Goodwill Industries of Central Florida INC | Health Care and Social Assistance | $5,862,500 | $5,880,739 | 500 | N/A | Research |
| Agmus Ventures Inc. | Finance and Insurance | $5,762,400 | $5,835,550 | 177 | $90.9M | Research |
| Aslan International Ministry, Inc. | Health Care and Social Assistance | $5,528,000 | $0 | 486 | $78.7M | Research |
| Central Florida Young Men's Christian Association | Arts, Entertainment, and Recreation | $5,370,891 | $4,169,392 | 500 | $70.0M | Research |
| Emergency Physicians of Central Florida, LLP | Health Care and Social Assistance | $5,254,522 | $5,016,042 | 241 | $75.6M | Research |
| Red Lobster Management LLC | Accommodation and Food Services | $5,222,997 | $5,254,621 | 295 | $52.4M | Research |
| Ust Prime III Hotel Owner, L.p. | Accommodation and Food Services | $5,006,899 | $4,590,004 | 343 | $107.3M | Research |
| Lake Highland Preparatory School INC | Educational Services | $4,864,100 | $4,919,091 | 425 | N/A | Research |