| Security First Managers, LLC | Finance and Insurance | $5,749,600 | $5,817,318 | 425 | $218.4M |  Research |
| Ladies Professional Golf Association INC | Arts, Entertainment, and Recreation | $1,990,000 | $1,999,541 | 124 | N/A | Research |
| Emergency Medicine Professionals, PA | Health Care and Social Assistance | $1,958,000 | $1,982,747 | 90 | $28.2M | Research |
| Germfree Laboratories Incorporated | Manufacturing | $1,785,699 | $1,807,326 | 155 | $43.3M | Research |
| S.r. Perrott INC | Wholesale Trade | $1,620,837 | $1,642,178 | 174 | $44.8M | Research |
| Haley Construction INC | Construction | $1,479,367 | $1,488,932 | 50 | $57.0M | Research |
| Halifax Paving, Inc. | Construction | $1,358,200 | $1,367,838 | 119 | $36.9M | Research |
| Microflex INC | Manufacturing | $1,283,800 | $1,297,530 | 120 | $31.4M | Research |
| Tomoka Eye Associates P.a. | Health Care and Social Assistance | $1,254,126 | $1,268,200 | 105 | $24.6M | Research |
| Coquina Center LLC DBA Coquina Center | Health Care and Social Assistance | $1,193,440 | $1,203,186 | 81 | $13.8M | Research |
| Bridgeview Center LLC DBA Bridgeview Center | Health Care and Social Assistance | $1,185,127 | $1,194,641 | 112 | $19.1M | Research |
| United Water Restoration Group INC | Administrative and Support and Waste Management | $1,044,644 | $724,861 | 100 | $21.7M | Research |
| Calvary Assembly of God of Ormond Beach, Florida, Inc. | Other Services (except Public Administration) | $981,300 | $988,478 | 130 | N/A | Research |
| Florida Family Foods LLC | Wholesale Trade | $979,956 | $990,400 | 197 | $50.7M | Research |
| Command Medical Products, Inc. | Manufacturing | $978,485 | $985,911 | 135 | $37.7M | Research |
| Rossmeyer Daytona Motorcycles, Inc. | Retail Trade | $971,200 | $980,992 | 74 | $19.3M | Research |
| Total Comfort Heat + Air Condi | Construction | $962,300 | $970,684 | 77 | $24.4M | Research |
| Haley Construction INC | Construction | $936,235 | $943,186 | 58 | $66.1M | Research |
| Rossmeyer Daytona Motorcycles INC | Retail Trade | $864,277 | $868,910 | 74 | $19.3M | Research |
| Cunningham Field and Research Service in | Professional, Scientific, and Technical Services | $816,953 | $822,616 | 138 | $19.3M | Research |
| Cunningham Field and Research Service in | Professional, Scientific, and Technical Services | $809,500 | $814,157 | 138 | $19.3M | Research |
| Nova King Crew, LLC | Unknown | $791,600 | $798,822 | 157 | N/A | Research |
| Sammarbra LLC | Accommodation and Food Services | $739,256 | $742,809 | 76 | $13.5M | Research |
| United Water Restoration Group INC | Construction | $716,207 | $722,839 | 50 | $17.0M | Research |
| Parks Dermatology Center, LLC | Health Care and Social Assistance | $705,200 | $711,537 | 64 | $11.7M | Research |