| Mitchell Management Company | Accommodation and Food Services | $1,088,800 | $1,098,750 | 184 | $32.7M | Research |
| Mitchell Management of Arizona, LLC | Accommodation and Food Services | $973,500 | $982,532 | 173 | $30.7M | Research |
| Mitchell Management of Florida Inc. | Accommodation and Food Services | $663,500 | $669,564 | 124 | $22.0M | Research |
| Crush-It INC | Administrative and Support and Waste Management | $601,484 | $609,236 | 62 | $11.7M | Research |
| Lucky Star Golf LLC | Arts, Entertainment, and Recreation | $538,600 | $0 | 85 | $13.9M | Research |
| Casey Key Fish House INC | Accommodation and Food Services | $441,000 | $336,861 | 25 | <$5M | Research |
| Pinnacle Express Delivery, LLC | Transportation and Warehousing | $426,511 | $431,173 | 95 | $26.1M | Research |
| Chef's Garden Restaurant Inc. | Accommodation and Food Services | $339,181 | $343,675 | 50 | $8.9M | Research |
| Osprey Leisure LLC | Accommodation and Food Services | $336,183 | $213,575 | 31 | $9.7M | Research |
| Insurance Service of Sarasota, Inc. | Finance and Insurance | $322,257 | $325,721 | 25 | $12.8M | Research |
| Casey Key Fish House INC | Accommodation and Food Services | $314,999 | $319,236 | 36 | $6.4M | Research |
| Mad Moe's LLC | Accommodation and Food Services | $289,247 | $290,800 | 31 | $5.5M | Research |
| Sarasota Skin and Cancer Center INC | Health Care and Social Assistance | $284,993 | $287,906 | 26 | $8.2M | Research |
| Justin Stiver | Health Care and Social Assistance | $267,486 | $269,846 | 21 | $5.7M | Research |
| Total Therapy Florida, LLC | Health Care and Social Assistance | $250,200 | $252,771 | 21 | <$5M | Research |
| Sarasota Home Health Care Agency, LLC | Health Care and Social Assistance | $247,186 | $248,811 | 46 | $7.9M | Research |
| Tommy's Express Holdings, Inc. | Other Services (except Public Administration) | $232,233 | $233,897 | 58 | $11.4M | Research |
| Osprey Leisure LLC | Accommodation and Food Services | $230,981 | $233,329 | 33 | $10.3M | Research |
| Chef's Garden Restaurant, Inc. | Accommodation and Food Services | $220,225 | $223,167 | 50 | $8.9M | Research |
| Mad Moe's LLC | Accommodation and Food Services | $206,606 | $208,174 | 25 | <$5M | Research |
| Braendel Painting INC | Construction | $190,082 | $191,867 | 25 | $6.3M | Research |
| Braendel Painting INC | Construction | $187,115 | $188,643 | 30 | $7.6M | Research |
| Stratapt,llc | Professional, Scientific, and Technical Services | $181,383 | $182,799 | 22 | <$5M | Research |
| Glueck's Auto Parts II Inc. | Retail Trade | $180,500 | $182,882 | 18 | <$5M | Research |
| The Conservation Foundation of the Gulf Coast | Professional, Scientific, and Technical Services | $162,956 | $164,848 | 9 | N/A | Research |