| Community Eye Center | Health Care and Social Assistance | $10,000,000 | $925,215 | 100 | $31.4M |  Research |
| Grallwaterproofing LLC | Construction | $1,592,400 | $1,605,990 | 186 | $63.4M | Research |
| Fuccillo Associates of Florida, Inc. | Retail Trade | $1,295,145 | $1,221,405 | 90 | $22.7M | Research |
| Port Charlotte Auto Retail, Inc. | Retail Trade | $1,139,700 | $1,155,434 | 119 | $30.0M | Research |
| Village Place Nh LLC | Health Care and Social Assistance | $1,064,692 | $1,078,606 | 114 | $19.4M | Research |
| Advanced Orthopedic Center of Charlotte County, P.a. | Health Care and Social Assistance | $990,023 | $998,552 | 82 | $25.7M | Research |
| Don Gasgart Ford, INC | Retail Trade | $913,100 | $920,024 | 81 | $20.4M | Research |
| Charlotte Heart and Vascular Institute PA | Health Care and Social Assistance | $851,100 | $859,304 | 75 | $23.5M | Research |
| King of the Road Mc LLC | Retail Trade | $841,317 | $852,127 | 54 | $14.1M | Research |
| Gasparilla Marine Sales, Inc. | Retail Trade | $769,800 | $779,944 | 68 | $17.8M | Research |
| Advocate Radiation Oncology, LLC | Health Care and Social Assistance | $723,700 | $729,192 | 42 | $13.2M | Research |
| Bacon Galleries, INC | Retail Trade | $710,500 | $718,092 | 72 | $13.1M | Research |
| Superior Pools of Southwest Florida INC | Construction | $623,164 | $637,317 | 50 | $17.0M | Research |
| First Baptist Church of Port Charlotte | Other Services (except Public Administration) | $510,300 | $514,005 | 55 | N/A | Research |
| Oaktree Consulting, LLC | Construction | $495,200 | $499,726 | 45 | $14.2M | Research |
| Cardiology Associates of Charlotte County PA | Health Care and Social Assistance | $485,609 | $490,093 | 38 | $11.9M | Research |
| Pulmonary, Sleep and Critical Care Specialists, P.l. | Health Care and Social Assistance | $480,700 | $485,204 | 38 | $11.9M | Research |
| Chapman Insurance Group, LLC | Finance and Insurance | $479,800 | $482,365 | 37 | $19.0M | Research |
| Galloway Roofing, LLC | Construction | $462,180 | $468,258 | 48 | $15.8M | Research |
| Swaroop Muppavarapu, M.d., P.a. | Health Care and Social Assistance | $454,775 | $459,492 | 10 | <$5M | Research |
| All Phase Custom Mill Shop, Inc. | Manufacturing | $449,985 | $338,083 | 45 | $11.8M | Research |
| Third Worthington INC | Unknown | $430,250 | $434,431 | 146 | N/A | Research |
| Innovative Marine Structures, LLC | Construction | $414,587 | $418,687 | 30 | $9.3M | Research |
| Innovative Marine Structure LLC | Construction | $414,587 | $417,892 | 34 | $10.5M | Research |
| Ductan Entertainment | Professional, Scientific, and Technical Services | $412,490 | $0 | 22 | <$5M | Research |