| B & M Precision INC | Manufacturing | $3,503,600 | $3,552,553 | 287 | $80.1M |  Research |
| Bde Florida LLC | Accommodation and Food Services | $2,221,045 | $2,246,279 | 500 | $88.8M | Research |
| Speedling Incorporated | Agriculture, Forestry, Fishing and Hunting | $1,299,072 | $1,309,500 | 128 | $13.1M | Research |
| Speedling Incorporated | Agriculture, Forestry, Fishing and Hunting | $1,299,072 | $1,175,633 | 118 | $12.1M | Research |
| Southshore Hospitality Management LLC | Accommodation and Food Services | $756,084 | $761,822 | 75 | $13.3M | Research |
| Southshore Hospitality Management LLC | Accommodation and Food Services | $632,730 | $553,643 | 98 | $17.4M | Research |
| Dimare Ruskin INC | Wholesale Trade | $581,258 | $585,765 | 91 | $23.4M | Research |
| Suarez Grading Enterprises, INC | Construction | $413,531 | $417,046 | 49 | $18.4M | Research |
| Welch Tennis Court, INC | Arts, Entertainment, and Recreation | $402,700 | $384,086 | 52 | $7.3M | Research |
| Suarez Grading Enterprises, INC | Construction | $376,650 | $380,944 | 35 | $10.6M | Research |
| Tri-Area Electrical Construction Inc. | Construction | $333,989 | $337,768 | 39 | $12.8M | Research |
| Tomatoes of Ruskin, Inc. | Agriculture, Forestry, Fishing and Hunting | $311,985 | $316,110 | 175 | $8.8M | Research |
| Sun City Groves, INC | Agriculture, Forestry, Fishing and Hunting | $288,000 | $291,109 | 27 | <$5M | Research |
| Kennco Manufacturing INC | Manufacturing | $272,200 | $274,310 | 27 | $7.1M | Research |
| Florida Home Partnership, Inc. | Health Care and Social Assistance | $244,200 | $247,666 | 17 | N/A | Research |
| Elite Building Services LLC | Construction | $242,480 | $245,230 | 19 | <$5M | Research |
| Elite Building Services LLC | Construction | $242,200 | $245,279 | 19 | <$5M | Research |
| Debilini INC | Health Care and Social Assistance | $226,875 | $28,376 | 90 | $15.4M | Research |
| Lanese & Associates, Inc. | Professional, Scientific, and Technical Services | $222,000 | $224,641 | 14 | <$5M | Research |
| First Baptist Church of Ruskin INC | Other Services (except Public Administration) | $202,245 | $203,823 | 40 | N/A | Research |
| Ybor Grille LLC | Accommodation and Food Services | $198,870 | $201,389 | 31 | $5.5M | Research |
| Apollo Construction and Engineering Serv | Construction | $189,862 | $192,525 | 9 | <$5M | Research |
| Lee Te Kim Lawn Care & Nursery INC | Retail Trade | $188,000 | $189,911 | 21 | <$5M | Research |
| Np Alpha Consulting, LLC | Professional, Scientific, and Technical Services | $187,378 | $188,292 | 30 | <$5M | Research |
| Shango Logistics Trucking LLC | Transportation and Warehousing | $183,793 | $0 | 12 | <$5M | Research |