| American Institute of Dermatology PA | Health Care and Social Assistance | $332,614 | $328,960 | 28 | $8.8M |  Research |
| Heartland Business Services INC | Professional, Scientific, and Technical Services | $322,756 | $324,295 | 66 | $21.0M | Research |
| Maxwell Medical, Pllc | Health Care and Social Assistance | $306,040 | $307,621 | 34 | $10.7M | Research |
| Mid-State Dewatering Equipment, Inc. | Construction | $299,535 | $301,244 | 40 | $13.6M | Research |
| Sebring Heart Center PA | Health Care and Social Assistance | $297,532 | $149,620 | 19 | <$5M | Research |
| William E. Chen, D.m.d., P.l. | Health Care and Social Assistance | $294,557 | $296,308 | 27 | $6.3M | Research |
| William E. Chen D.m.d. P.l. | Health Care and Social Assistance | $286,198 | $288,229 | 30 | $7.0M | Research |
| Fellowship Home at the Fairway LLC | Health Care and Social Assistance | $284,295 | $287,995 | 57 | $9.7M | Research |
| New Spring Lake Partners, LLC | Accommodation and Food Services | $282,953 | $284,556 | 40 | $12.5M | Research |
| Dental Care of Mid Florida, P.a. | Health Care and Social Assistance | $279,000 | $282,333 | 28 | $6.5M | Research |
| Chicanes Inc. | Accommodation and Food Services | $277,333 | $278,866 | 25 | <$5M | Research |
| I-O-T-L Inc. | Accommodation and Food Services | $269,605 | $271,095 | 30 | $9.4M | Research |
| Sunshine Staffing of Highlands County INC | Administrative and Support and Waste Management | $268,559 | $270,104 | 48 | $7.6M | Research |
| Alan Jay Nissan, Inc. | Retail Trade | $264,086 | $266,265 | 22 | $5.5M | Research |
| Alan Jay Nissan INC | Retail Trade | $264,069 | $265,022 | 22 | $5.5M | Research |
| Heartland Business Services, Inc. | Professional, Scientific, and Technical Services | $259,345 | $261,132 | 50 | $15.9M | Research |
| Hidden Acres Nursery, Inc. | Retail Trade | $247,253 | $248,627 | 38 | $7.8M | Research |
| Ozblu USA LLC | Agriculture, Forestry, Fishing and Hunting | $243,210 | $246,162 | 34 | <$5M | Research |
| Sunny Hills Alf of Sebring, Inc. | Health Care and Social Assistance | $240,075 | $0 | 48 | $8.2M | Research |
| All About Lawns, Inc. | Administrative and Support and Waste Management | $238,000 | $240,015 | 26 | $5.6M | Research |
| All About Lawns Inc. | Administrative and Support and Waste Management | $238,000 | $238,945 | 26 | $5.6M | Research |
| New Spring Lake Partners, LLC | Arts, Entertainment, and Recreation | $237,666 | $239,096 | 35 | <$5M | Research |
| Hidden Acres Nursery, Inc. | Agriculture, Forestry, Fishing and Hunting | $235,486 | $236,402 | 33 | <$5M | Research |
| Ozblu USA LLC | Agriculture, Forestry, Fishing and Hunting | $233,223 | $235,267 | 33 | <$5M | Research |
| Mcs Contracting, Inc. | Construction | $233,170 | $234,621 | 20 | $22.8M | Research |