| Jts Enterprises of Tampa Ltd | Accommodation and Food Services | $10,000,000 | $10,138,889 | 500 | $88.8M |  Research |
| Truecore Behavioral Solutions, LLC | Health Care and Social Assistance | $10,000,000 | $10,084,110 | 500 | $81.0M | Research |
| Lutheran Services Florida, INC | Other Services (except Public Administration) | $10,000,000 | $5,716,721 | 500 | N/A | Research |
| Oracle Elevator Holdco, Inc. | Other Services (except Public Administration) | $8,421,400 | $8,329,648 | 499 | $107.2M | Research |
| Rls (usa) Inc. | Manufacturing | $8,193,698 | $8,247,867 | 490 | $486.0M | Research |
| System Soft Technologies LLC | Professional, Scientific, and Technical Services | $7,774,106 | $7,102,633 | 384 | $79.4M | Research |
| Bisk Education Inc. | Public Administration | $7,712,701 | $7,811,466 | 439 | N/A | Research |
| Agilethought LLC | Professional, Scientific, and Technical Services | $7,590,117 | $7,405,104 | 326 | $45.6M | Research |
| Ardurra Group, INC | Professional, Scientific, and Technical Services | $7,359,755 | $7,445,006 | 438 | $54.0M | Research |
| Hsp Southern Healthcare LLC | Health Care and Social Assistance | $7,300,000 | $7,438,600 | 499 | $84.9M | Research |
| Gardner-Gibson INC | Manufacturing | $6,261,200 | $6,329,473 | 443 | $439.4M | Research |
| Physician Partners of America LLC | Management of Companies and Enterprises | $5,978,709 | $6,109,258 | 403 | $66.2M | Research |
| Healthesystems LLC | Information | $5,934,400 | $6,003,470 | 250 | $42.0M | Research |
| Phase Three Star, LLC DBA Hardee's | Unknown | $5,739,795 | $6,362,785 | 500 | N/A | Research |
| Keenan, Hopkins, Schmidt Stowell Contractors INC | Construction | $5,581,000 | $5,662,080 | 307 | $349.8M | Research |
| Morgan & Morgan, Tampa, P.a. | Professional, Scientific, and Technical Services | $5,518,400 | $5,591,576 | 469 | $114.0M | Research |
| Hillcour INC | Management of Companies and Enterprises | $5,424,015 | $5,520,138 | 353 | $58.0M | Research |
| Tampa Bay Systems Sales, Inc. | Construction | $5,400,000 | $5,458,950 | 310 | $98.1M | Research |
| Avesta Homes LLC | Real Estate and Rental and Leasing | $5,221,600 | $4,224,844 | 277 | $41.5M | Research |
| Butler Weihmuller Katz Craig LLP | Professional, Scientific, and Technical Services | $5,214,435 | $5,271,008 | 296 | $71.9M | Research |
| Mcnichols Company | Wholesale Trade | $5,045,200 | $5,104,360 | 250 | $76.6M | Research |
| Pepin Distributing Company | Transportation and Warehousing | $4,900,000 | $4,970,369 | 367 | $98.0M | Research |
| Electrostim Medical Services Inc. | Wholesale Trade | $4,810,000 | $4,887,227 | 374 | $114.6M | Research |
| Kisinger Campo & Associates, Corp | Professional, Scientific, and Technical Services | $4,764,100 | $0 | 285 | $35.1M | Research |
| Wob Holdings, LLC | Accommodation and Food Services | $4,724,992 | $4,787,906 | 77 | $13.7M | Research |