| Maddox Electric Company INC | Construction | $4,113,100 | $4,158,513 | 239 | $78.6M |  Research |
| Kelly's Food, Inc. | Wholesale Trade | $2,100,800 | $1,821,709 | 234 | $60.2M | Research |
| Heichel Plumbing, Inc. | Construction | $2,007,600 | $2,030,646 | 175 | $55.4M | Research |
| Maddox Electric Company Inc. | Construction | $2,000,000 | $2,018,849 | 123 | $40.5M | Research |
| Paul Pimentel DDS Holdings, Pllc | Health Care and Social Assistance | $1,873,745 | $1,892,688 | 139 | $32.2M | Research |
| Florida Paints and Coatings LLC | Manufacturing | $1,855,200 | $1,865,416 | 163 | $161.7M | Research |
| Armstrong Air Condition & Heating of Central Florida Inc. | Construction | $1,749,112 | $1,758,648 | 178 | $56.3M | Research |
| Vmg Construction INC | Construction | $1,625,436 | $1,637,237 | 162 | $184.6M | Research |
| Rapid Building Solutions LLC | Construction | $1,580,875 | $1,594,664 | 160 | $54.5M | Research |
| Rapid Building Solutions, LLC | Professional, Scientific, and Technical Services | $1,580,875 | $1,593,097 | 178 | $22.0M | Research |
| Armstrong Air Condition & Heating of Central Florida, Inc. | Construction | $1,428,000 | $1,441,967 | 176 | $55.7M | Research |
| Schmid Construction Inc. | Construction | $1,412,500 | $1,424,506 | 65 | $74.1M | Research |
| Johnson-Laux Construction LLC | Construction | $1,280,072 | $1,288,037 | 73 | $83.2M | Research |
| Contech Construction LLC | Construction | $1,276,100 | $1,276,269 | 70 | $79.8M | Research |
| Contech Construction, LLC | Construction | $1,276,100 | $1,283,722 | 59 | $67.2M | Research |
| Thermo King of the Southeast, LLC | Retail Trade | $1,245,386 | $1,252,617 | 93 | $24.3M | Research |
| Hanks, Hanks and Associates, LLC | Professional, Scientific, and Technical Services | $1,241,200 | $1,247,992 | 150 | $15.5M | Research |
| Vmg Construction INC | Construction | $1,215,700 | $1,222,353 | 151 | $34.9M | Research |
| Johnson-Laux Construction, LLC | Construction | $1,208,156 | $1,217,284 | 67 | $76.3M | Research |
| Solazzo Electrical Contractors, Inc. | Construction | $1,199,200 | $15,579 | 3 | <$5M | Research |
| Foundation Academy of Winter Garden INC | Educational Services | $1,186,715 | $1,195,689 | 173 | N/A | Research |
| Gibbs & Register INC | Construction | $1,131,932 | $1,138,692 | 82 | $25.4M | Research |
| International Service Contractors LLC | Professional, Scientific, and Technical Services | $1,052,512 | $1,064,294 | 151 | $15.6M | Research |
| Knox Horticulture | Agriculture, Forestry, Fishing and Hunting | $1,047,900 | $1,058,670 | 150 | $15.3M | Research |
| Winter Garden Rehab, LLC | Health Care and Social Assistance | $1,043,624 | $1,054,437 | 125 | $21.3M | Research |