| Indian River Transport Co | Transportation and Warehousing | $5,500,000 | $5,551,534 | 479 | $109.2M |  Research |
| Central Florida Healthcare INC | Health Care and Social Assistance | $4,350,000 | $4,399,783 | 408 | N/A | Research |
| Bond & Steele Clinic, P.a. | Health Care and Social Assistance | $4,243,070 | $4,292,011 | 497 | $156.0M | Research |
| Gessler Clinic Professional Association | Health Care and Social Assistance | $3,204,382 | $3,240,965 | 283 | $88.8M | Research |
| Tucker Paving INC | Construction | $2,074,790 | $2,091,965 | 154 | $47.7M | Research |
| Spring Lake Nc, LLC | Health Care and Social Assistance | $2,012,700 | $1,830,238 | 281 | $47.8M | Research |
| Commercial Warehousing INC | Transportation and Warehousing | $1,893,328 | $1,908,264 | 208 | $52.8M | Research |
| Eye Specialists of Mid-Florida P a | Health Care and Social Assistance | $1,791,823 | $1,807,402 | 188 | $59.0M | Research |
| Certipay America, LLC | Professional, Scientific, and Technical Services | $1,751,428 | $1,766,509 | 136 | $43.3M | Research |
| Help Eye Specialists of Mid Florida PA and Da | Health Care and Social Assistance | $1,659,400 | $1,676,865 | 207 | $65.0M | Research |
| Clear Springs Enterprises, LLC | Wholesale Trade | $1,572,537 | $1,530,203 | 209 | $53.8M | Research |
| Wm. G. Roe & Sons Inc. | Manufacturing | $1,398,520 | $1,419,459 | 127 | $44.8M | Research |
| Wm. G. Roe & Sons, Inc. & Subsidiaries | Wholesale Trade | $1,292,000 | $1,303,915 | 174 | $44.8M | Research |
| Jarrett Gordon Ford Lincoln, Inc. | Retail Trade | $1,189,353 | $1,197,348 | 96 | $24.2M | Research |
| Raysway, Inc. | Accommodation and Food Services | $1,166,300 | $1,177,542 | 445 | $79.0M | Research |
| Brandywine Convalescent Center Inc. | Health Care and Social Assistance | $1,076,168 | $1,088,345 | 124 | $21.1M | Research |
| Vitreous and Retina Consultants PA | Health Care and Social Assistance | $1,076,000 | $1,087,806 | 71 | $22.3M | Research |
| Peterson & Myers, P.a | Professional, Scientific, and Technical Services | $1,051,300 | $1,062,280 | 62 | $15.1M | Research |
| All Saints Academy | Educational Services | $1,011,850 | $1,017,062 | 85 | N/A | Research |
| The Barranco Clinic, P.a. | Health Care and Social Assistance | $972,000 | $977,585 | 64 | $20.1M | Research |
| The Tinsley Family Concessions Inc. | Accommodation and Food Services | $923,947 | $934,880 | 98 | $17.4M | Research |
| K. Wade Foster MD PA | Health Care and Social Assistance | $878,652 | $886,291 | 96 | $30.1M | Research |
| Chevrolet Center, Inc. | Wholesale Trade | $843,975 | $498,255 | 72 | $22.1M | Research |
| Winter Haven Ventures, Inc. | Wholesale Trade | $824,072 | $830,665 | 78 | $23.9M | Research |
| Weber Environmental Services Inc. | Professional, Scientific, and Technical Services | $787,300 | $794,342 | 110 | $22.2M | Research |