| Advanced It Concepts INC | Professional, Scientific, and Technical Services | $2,250,078 | $2,175,342 | 152 | $31.4M | Research |
| Lake Doctors Inc, the | Agriculture, Forestry, Fishing and Hunting | $1,569,148 | $1,578,735 | 140 | $7.0M | Research |
| Ohio Alpha Environmental Management Corporation | Professional, Scientific, and Technical Services | $1,561,428 | $1,577,953 | 193 | $20.0M | Research |
| Modern Plumbing Industries, Inc. | Construction | $1,529,000 | $1,544,715 | 135 | $42.7M | Research |
| Prosperity Life II LLC | Professional, Scientific, and Technical Services | $987,622 | $0 | 55 | $6.2M | Research |
| Reiss Engineering, Inc. | Professional, Scientific, and Technical Services | $860,200 | $866,245 | 36 | <$5M | Research |
| Alpha Environmental Management Corp, LLC | Professional, Scientific, and Technical Services | $833,150 | $842,685 | 112 | $11.6M | Research |
| Irvin Technologies, Inc. | Professional, Scientific, and Technical Services | $681,400 | $689,804 | 1 | <$5M | Research |
| Epic Engineering & Consulting Group LLC | Professional, Scientific, and Technical Services | $639,910 | $646,078 | 33 | <$5M | Research |
| Epic Engineering & Consulting Group, LLC | Professional, Scientific, and Technical Services | $639,615 | $644,412 | 35 | <$5M | Research |
| Unlimited Welding Inc. | Construction | $587,352 | $592,612 | 26 | $8.9M | Research |
| H.l. Pruitt Corp | Retail Trade | $579,500 | $583,485 | 45 | $7.0M | Research |
| Mercon Construction Company | Construction | $532,151 | $536,131 | 45 | $51.3M | Research |
| Mercon Construction Co INC | Construction | $523,182 | $526,851 | 45 | $51.3M | Research |
| Mid Florida Hospital Specialists PA | Health Care and Social Assistance | $513,300 | $624,402 | 32 | $10.0M | Research |
| Roof Top Services of Central Florida, Inc. | Construction | $476,403 | $483,334 | 22 | $7.3M | Research |
| Unlimited Welding, Inc. | Manufacturing | $473,250 | $476,471 | 420 | $102.7M | Research |
| B&d Waterblasting Co INC | Construction | $425,500 | $430,221 | 32 | $9.7M | Research |
| A Budget Tree Service, Inc. | Administrative and Support and Waste Management | $416,473 | $363,229 | 46 | $10.0M | Research |
| Professional Imaging Centers INC | Health Care and Social Assistance | $405,828 | $409,341 | 48 | $11.1M | Research |
| Professional Imaging Centers INC | Health Care and Social Assistance | $405,827 | $408,785 | 48 | $11.1M | Research |
| Southern Marketing Associates INC | Retail Trade | $378,000 | $381,542 | 18 | <$5M | Research |
| Rhine Enterprises INC | Accommodation and Food Services | $372,100 | $375,077 | 127 | $22.5M | Research |
| F.h. Collins III DDS P.a. DBA Collins Dental | Health Care and Social Assistance | $363,200 | $366,822 | 30 | $7.0M | Research |
| Walt Dittmer & Sons, Inc. | Construction | $351,500 | $355,376 | 32 | $10.9M | Research |