| Schuster Enterprises, Inc. | Accommodation and Food Services | $10,000,000 | $10,086,027 | 500 | $88.8M | Research |
| Management Services Network LLC | Professional, Scientific, and Technical Services | $4,422,512 | $4,473,159 | 475 | $49.2M | Research |
| Hmmg, LLC | Health Care and Social Assistance | $4,323,752 | $4,373,623 | 435 | $136.5M | Research |
| Peek Pavement Marking, LLC | Construction | $4,187,643 | $4,236,403 | 325 | $100.7M | Research |
| The Concrete Company | Manufacturing | $3,952,877 | $3,999,012 | 400 | $396.7M | Research |
| Gfmco LLC | Manufacturing | $3,333,638 | $3,371,449 | 242 | $63.4M | Research |
| Pezold Management Associates, Inc. | Management of Companies and Enterprises | $3,234,468 | $3,282,055 | 500 | $82.2M | Research |
| Standard Concrete Products, Inc. | Construction | $3,099,650 | $3,134,383 | 278 | $64.3M | Research |
| Lauderdale Dental, LLC | Health Care and Social Assistance | $2,733,271 | $667,908 | 161 | $37.3M | Research |
| Valley Hospitality Services LLC | Accommodation and Food Services | $2,097,228 | $2,127,508 | 419 | $131.0M | Research |
| Staffing Connections, LLC | Administrative and Support and Waste Management | $2,047,613 | $2,070,220 | 329 | $51.8M | Research |
| Alexander Electric Co | Construction | $2,045,300 | $2,069,503 | 201 | $66.1M | Research |
| Gfmco LLC | Manufacturing | $2,000,000 | $2,018,247 | 237 | $62.1M | Research |
| Valley Hospitality Services LLC | Accommodation and Food Services | $2,000,000 | $2,019,671 | 166 | $51.9M | Research |
| The Brookstone School INC | Educational Services | $1,968,343 | $1,987,433 | 323 | $38.8M | Research |
| Columbus Hospice Inc. | Health Care and Social Assistance | $1,711,313 | $1,719,939 | 156 | $26.7M | Research |
| Teen Challenge of Florida, Inc. | Other Services (except Public Administration) | $1,569,568 | $1,585,220 | 221 | $42.2M | Research |
| St Luke United Methodist Church | Other Services (except Public Administration) | $1,512,988 | $1,532,180 | 19 | N/A | Research |
| Rkj & Sons LLC | Accommodation and Food Services | $1,503,968 | $1,519,718 | 252 | $44.7M | Research |
| St Luke United Methodist Church | Other Services (except Public Administration) | $1,500,000 | $1,514,137 | 252 | N/A | Research |
| Human Performance & Rehabilitation Centers INC | Health Care and Social Assistance | $1,431,750 | $1,444,577 | 139 | $37.5M | Research |
| Woodruff Property Management Company | Real Estate and Rental and Leasing | $1,277,075 | $1,284,318 | 123 | $18.4M | Research |
| Jordan Outdoor Enterprises Ltd | Real Estate and Rental and Leasing | $1,273,500 | $1,283,157 | 60 | $9.0M | Research |
| Robinson, Grimes & Company PC | Professional, Scientific, and Technical Services | $1,253,091 | $1,264,111 | 61 | $19.4M | Research |
| A-Com Enterprises, Inc. | Administrative and Support and Waste Management | $1,163,158 | $1,169,755 | 101 | $14.8M | Research |