| The Hospital Authority of Wayne County, Ga | Health Care and Social Assistance | $5,094,187 | $5,152,912 | 433 | $85.9M |  Research |
| Universal Electric Co. Inc. | Construction | $1,957,513 | $1,970,781 | 93 | $30.6M | Research |
| Universal Electric Company, Inc. | Construction | $1,957,507 | $1,978,496 | 120 | $39.5M | Research |
| Boykin Erectors, Inc. | Construction | $968,691 | $975,418 | 65 | $22.1M | Research |
| Boykin Erectorsinc. | Construction | $949,485 | $954,206 | 72 | $16.7M | Research |
| Ronofry LLC | Construction | $895,600 | $905,029 | 25 | $9.4M | Research |
| Airco Industrial Services LLC | Construction | $865,866 | $870,725 | 50 | $15.9M | Research |
| Bone & Joint Institute of South Georgia, PC | Health Care and Social Assistance | $758,020 | $767,622 | 79 | $24.8M | Research |
| Harborview Jesup LLC | Health Care and Social Assistance | $682,442 | $691,491 | 104 | $17.7M | Research |
| Bone & Joint Institute of South Georgia PC | Health Care and Social Assistance | $671,716 | $681,083 | 92 | $28.9M | Research |
| Mcdaniel Supply Company, Inc. | Retail Trade | $563,600 | $567,341 | 70 | $10.9M | Research |
| Mobile Concrete, Inc. | Construction | $471,569 | $477,097 | 49 | $11.3M | Research |
| Raymond M. Harris & Co., Inc. DBA Harris Ace Hardware | Retail Trade | $458,500 | $462,193 | 73 | $15.1M | Research |
| Nesmith Chevrolet Buick Gmc of Jesup, Inc. | Retail Trade | $454,900 | $457,920 | 39 | $9.8M | Research |
| Nesmith Chevrolet Buick Gmc of Jesup INC | Retail Trade | $442,033 | $444,636 | 36 | $9.1M | Research |
| Wayne County Anesthesia Associates PC | Health Care and Social Assistance | $391,200 | $135,964 | 9 | <$5M | Research |
| Hendrix Hauling Company Inc. | Transportation and Warehousing | $373,414 | $375,219 | 48 | $9.7M | Research |
| Hendrix Hauling Company | Transportation and Warehousing | $348,041 | $351,280 | 50 | $13.3M | Research |
| Family Tradition Steak House Inc. | Accommodation and Food Services | $329,364 | $331,130 | 35 | $6.2M | Research |
| First Choise Concrete, LLC | Construction | $290,851 | $292,313 | 37 | $8.6M | Research |
| Carter Dredging Ii, INC | Mining, Quarrying, and Oil and Gas Extraction | $268,900 | $272,545 | 21 | <$5M | Research |
| Larry Williams | Accommodation and Food Services | $266,584 | $267,739 | 55 | $9.8M | Research |
| Family Tradition Steak House, Inc. | Accommodation and Food Services | $235,260 | $236,926 | 65 | $11.5M | Research |
| Rwp Incorporated | Manufacturing | $221,112 | $222,598 | 28 | $27.8M | Research |
| Wolfe Animal Hospital, P.c. | Professional, Scientific, and Technical Services | $203,700 | $205,067 | 25 | <$5M | Research |