| Innovative Staff Solutions, Inc. | Administrative and Support and Waste Management | $10,000,000 | $10,166,667 | 500 | $78.7M |  Research |
| Mattoon Precision Manufacturing, Inc. | Manufacturing | $2,402,700 | $2,429,530 | 171 | $44.8M | Research |
| Curry Construction, Inc. | Construction | $2,156,900 | $2,182,133 | 130 | $148.1M | Research |
| Heartland Health System Inc. | Health Care and Social Assistance | $1,850,000 | $1,872,303 | 325 | $64.5M | Research |
| Kc Summers, Inc. | Retail Trade | $1,552,790 | $1,562,873 | 129 | $32.5M | Research |
| Anamet Electrical, Inc. | Manufacturing | $1,526,200 | $1,543,031 | 110 | $28.8M | Research |
| Dan Pilson Auto Center Inc. | Retail Trade | $1,462,300 | $1,473,348 | 144 | $36.3M | Research |
| Alliance Tractor LLC | Retail Trade | $1,421,800 | $1,389,294 | 116 | $23.9M | Research |
| West & Company LLC | Professional, Scientific, and Technical Services | $1,093,200 | $1,099,759 | 76 | $24.2M | Research |
| Commercial Electric, INC | Construction | $949,100 | $954,979 | 48 | $15.8M | Research |
| Independent Order of Oddfellows | Health Care and Social Assistance | $946,992 | $952,332 | 131 | N/A | Research |
| Coles-Moultrie Electric Cooperative | Utilities | $798,979 | $805,792 | 34 | N/A | Research |
| Coles County Mental Health Association, Inc. | Health Care and Social Assistance | $563,100 | $570,092 | 78 | N/A | Research |
| Innovative Recruiting Solutions, Inc. | Administrative and Support and Waste Management | $429,993 | $50,486 | 28 | <$5M | Research |
| American Select Tubing, LLC | Wholesale Trade | $405,400 | $407,990 | 31 | $9.5M | Research |
| Lanman Oil Co. Inc. | Wholesale Trade | $379,398 | $380,652 | 65 | $16.7M | Research |
| Lanman Oil Company, Inc. | Retail Trade | $352,400 | $354,769 | 48 | $16.1M | Research |
| Doehring, Winders & Co LLP | Professional, Scientific, and Technical Services | $341,155 | $343,791 | 24 | $7.6M | Research |
| Restoration Dental, LLC | Health Care and Social Assistance | $337,000 | $338,818 | 39 | $9.0M | Research |
| Restoration Dental LLC | Health Care and Social Assistance | $329,585 | $332,574 | 41 | $9.5M | Research |
| Ronchetti Distributing Co. | Wholesale Trade | $324,500 | $326,564 | 22 | $5.7M | Research |
| The Upchurch Group, Inc. | Professional, Scientific, and Technical Services | $300,800 | $302,956 | 22 | $6.8M | Research |
| Preston-Hunter, INC | Administrative and Support and Waste Management | $287,700 | $290,273 | 23 | <$5M | Research |
| Mattoon Area Young Mens Christian Association | Arts, Entertainment, and Recreation | $279,500 | $281,713 | 200 | N/A | Research |
| Mattoon Area Young Mens Christian Association | Other Services (except Public Administration) | $279,484 | $281,425 | 156 | N/A | Research |