| Heyl, Royster, Voelker & Allen PC | Professional, Scientific, and Technical Services | $4,864,800 | $4,921,826 | 276 | $67.1M |  Research |
| Oncology Hematology Associates of Central Illinois, PC | Health Care and Social Assistance | $4,800,000 | $0 | 454 | $142.5M | Research |
| The Children's Home Association of Illinois | Health Care and Social Assistance | $4,103,300 | $1,214,033 | 429 | N/A | Research |
| Connor Co | Wholesale Trade | $3,930,000 | $3,976,614 | 273 | $83.6M | Research |
| The Center for Youth and Family Solutions | Health Care and Social Assistance | $3,625,400 | $3,460,130 | 412 | N/A | Research |
| Dental Arts Laboratories INC | Manufacturing | $3,500,000 | $3,425,960 | 370 | $103.3M | Research |
| Wyman-Sicher Eye Associates, S.c. | Health Care and Social Assistance | $2,647,100 | $2,678,203 | 186 | $58.4M | Research |
| Petersen Health Operations LLC | Health Care and Social Assistance | $2,623,600 | $2,672,909 | 470 | $80.0M | Research |
| Alcast Company | Manufacturing | $2,588,125 | $2,562,234 | 163 | $42.7M | Research |
| Cgn & Associates, Inc. | Professional, Scientific, and Technical Services | $2,399,100 | $2,427,023 | 118 | $12.2M | Research |
| Petersen Management Company LLC | Health Care and Social Assistance | $2,351,600 | $2,376,082 | 496 | $84.4M | Research |
| The Pipco Companies, Ltd. | Construction | $2,245,110 | $2,272,114 | 110 | $34.8M | Research |
| Clark Management, LLC | Accommodation and Food Services | $2,240,700 | $2,267,339 | 500 | $88.8M | Research |
| Peoria Manpower, Inc. | Administrative and Support and Waste Management | $2,230,100 | $1,917,417 | 500 | $78.7M | Research |
| Midwest Orthopaedic Centers, S. C. | Health Care and Social Assistance | $2,227,600 | $2,254,269 | 166 | $52.1M | Research |
| Epic | Health Care and Social Assistance | $2,184,700 | $2,087,398 | 361 | N/A | Research |
| Mid-Illinois Companies, Corp. | Construction | $2,069,200 | $2,093,341 | 82 | $20.7M | Research |
| Alcast Company | Manufacturing | $2,000,000 | $2,006,833 | 163 | $42.7M | Research |
| Cgn & Associates, Inc. | Professional, Scientific, and Technical Services | $2,000,000 | $2,020,889 | 70 | $7.2M | Research |
| Wyman-Sicher Eye Associates S.c. | Health Care and Social Assistance | $2,000,000 | $2,018,111 | 186 | $58.4M | Research |
| Heartland Community Health Clinic | Health Care and Social Assistance | $1,900,000 | $1,918,894 | 180 | N/A | Research |
| Mercedes Restaurants Inc. | Accommodation and Food Services | $1,893,073 | $1,903,446 | 118 | $21.0M | Research |
| Laser Electric Inc. | Construction | $1,773,600 | $1,793,037 | 55 | $18.1M | Research |
| J.a.k. Enterprises, INC | Health Care and Social Assistance | $1,717,400 | $1,732,363 | 173 | $40.5M | Research |
| Lucwork Enterprises INC | Accommodation and Food Services | $1,652,000 | $1,672,466 | 264 | $46.9M | Research |