| Midwest Oilseeds, INC | Manufacturing | $1,177,500 | $1,185,146 | 79 | $27.9M |  Research |
| Stine Seed Company | Wholesale Trade | $973,700 | $979,782 | 65 | $16.7M | Research |
| Iowa Spring Manufacturing and Sales, INC | Manufacturing | $948,540 | $955,375 | 117 | $28.6M | Research |
| Kuder Inc. | Professional, Scientific, and Technical Services | $911,097 | $915,890 | 46 | $6.4M | Research |
| Kuder, Inc. | Educational Services | $830,587 | $835,047 | 51 | $6.6M | Research |
| Mertec, LLC | Manufacturing | $662,600 | $668,790 | 45 | $15.9M | Research |
| Inland Coatings Holdings LLC | Manufacturing | $654,900 | $658,542 | 38 | $37.7M | Research |
| Stine Seed Farm INC | Agriculture, Forestry, Fishing and Hunting | $625,800 | $629,863 | 42 | <$5M | Research |
| Eden Enterprise, INC | Manufacturing | $624,200 | $628,253 | 42 | $41.7M | Research |
| Valo Biomedia North America LLC | Agriculture, Forestry, Fishing and Hunting | $559,544 | $565,216 | 70 | $11.5M | Research |
| M.s. Technologies | Manufacturing | $536,200 | $541,959 | 36 | $12.7M | Research |
| Monarch Materials Group, INC | Retail Trade | $483,000 | $485,804 | 41 | $8.5M | Research |
| Vencomatic, Inc. | Transportation and Warehousing | $288,200 | $290,978 | 16 | <$5M | Research |
| Adel Healthcare Management, LLC | Health Care and Social Assistance | $272,000 | $274,252 | 45 | $7.7M | Research |
| Ew Nutrition Usa, Inc. | Manufacturing | $265,100 | $267,368 | 16 | $5.6M | Research |
| Neu Mannagement Pork, Inc. | Agriculture, Forestry, Fishing and Hunting | $255,336 | $256,648 | 27 | <$5M | Research |
| Hawkeye Breeders Service Inc. | Agriculture, Forestry, Fishing and Hunting | $246,928 | $251,107 | 27 | <$5M | Research |
| Crisis Intervention and Advocacy Center | Health Care and Social Assistance | $221,600 | $223,533 | 18 | N/A | Research |
| Crisis Intervention & Advocacy Center | Other Services (except Public Administration) | $221,600 | $223,945 | 18 | N/A | Research |
| Bow and Arrow Communications, Inc. | Administrative and Support and Waste Management | $210,672 | $211,855 | 10 | <$5M | Research |
| West Worldwide Services, Inc. | Manufacturing | $208,045 | $210,479 | 15 | $14.9M | Research |
| Core Physical Therapy, Inc. | Health Care and Social Assistance | $202,493 | $203,623 | 17 | <$5M | Research |
| Bow and Arrow Communications, Inc. | Administrative and Support and Waste Management | $183,573 | $184,443 | 11 | <$5M | Research |
| Cheesman Seed Company | Agriculture, Forestry, Fishing and Hunting | $174,300 | $175,360 | 12 | <$5M | Research |
| West Worldwide Services Inc. | Wholesale Trade | $171,934 | $173,314 | 15 | <$5M | Research |