| Amana Society Inc. | Retail Trade | $1,662,815 | $1,675,702 | 173 | $41.3M |  Research |
| Ox Yoke Inn, Inc. | Accommodation and Food Services | $470,523 | $473,973 | 49 | $8.7M | Research |
| Colonial Manor of Amana Inc. | Health Care and Social Assistance | $469,071 | $471,521 | 48 | $8.2M | Research |
| Colonial Manor of Amana, Inc. | Health Care and Social Assistance | $459,103 | $462,864 | 48 | $8.2M | Research |
| Ox Yoke Inn, Inc. | Accommodation and Food Services | $336,090 | $338,293 | 68 | $12.1M | Research |
| Amana Family Practice Clinic, P.c. | Health Care and Social Assistance | $188,903 | $190,041 | 21 | $6.6M | Research |
| Millstream Investments, INC | Manufacturing | $151,400 | $152,470 | 19 | $6.7M | Research |
| Essence of Life LLC | Health Care and Social Assistance | $141,400 | $142,449 | 24 | <$5M | Research |
| The Ronneburg Restaurant & Gifts INC | Accommodation and Food Services | $140,000 | $140,921 | 45 | $8.0M | Research |
| Millstream Investments INC | Accommodation and Food Services | $128,935 | $129,645 | 17 | <$5M | Research |
| The Ronneburg Restaurant & Gifts INC | Accommodation and Food Services | $125,200 | $126,418 | 55 | $9.8M | Research |
| John C Wagner Law Offices, P.c. | Professional, Scientific, and Technical Services | $105,680 | $106,456 | 8 | <$5M | Research |
| The Old Creamery Theatre Company | Arts, Entertainment, and Recreation | $90,992 | $91,428 | 25 | <$5M | Research |
| Kahler Golf LLC D/b/a Amana Colonies Golf Club | Arts, Entertainment, and Recreation | $76,600 | $77,057 | 5 | <$5M | Research |
| Kahler Golf LLC D/b/a Amana Colonies Golf Club | Arts, Entertainment, and Recreation | $73,200 | $73,716 | 50 | $7.0M | Research |
| Thompson Aero, Inc. | Agriculture, Forestry, Fishing and Hunting | $62,750 | $63,165 | 3 | <$5M | Research |
| Thompson Aero Inc. | Manufacturing | $59,774 | $59,935 | 3 | <$5M | Research |
| Iowa Valley Resource Conservation and Development | Public Administration | $57,545 | $57,815 | 7 | N/A | Research |
| Oehl Plumbing Heating Electrical & Ac | Construction | $55,457 | $55,990 | 5 | <$5M | Research |
| Iowa Valley Resource Conservation and Development | Public Administration | $55,312 | $55,724 | 8 | N/A | Research |
| Lakeview Village, INC | Health Care and Social Assistance | $52,920 | $53,315 | 7 | <$5M | Research |
| Uthoff Family Chiropractic PC | Health Care and Social Assistance | $48,510 | $48,775 | 4 | <$5M | Research |
| Uthoff Family Chiropractic P.c. | Health Care and Social Assistance | $48,510 | $48,874 | 4 | <$5M | Research |
| Christensen Family Dental, Plc | Health Care and Social Assistance | $41,442 | $41,751 | 3 | <$5M | Research |
| Zinser Wrecking Company, LLC | Construction | $36,100 | $36,292 | 3 | <$5M | Research |