| Boone County Hospital | Health Care and Social Assistance | $3,780,684 | $3,824,687 | 430 | N/A |  Research |
| Eastern Star Masonic Home, INC | Health Care and Social Assistance | $1,233,062 | $1,240,495 | 189 | N/A | Research |
| Eastern Star Masonic Home, INC | Health Care and Social Assistance | $1,229,900 | $1,238,783 | 197 | N/A | Research |
| Westhaven Community 'a Ministry of Evangelical Free Church' | Health Care and Social Assistance | $774,442 | $778,767 | 128 | N/A | Research |
| Kruck Plumbing & Heating Co. Inc. | Construction | $646,025 | $649,506 | 53 | $16.8M | Research |
| Redeker Furniture Co. | Retail Trade | $610,020 | $613,578 | 75 | $13.6M | Research |
| Kruck Plumbing & Heating Co, Inc. | Construction | $588,032 | $591,756 | 54 | $17.1M | Research |
| Mid-States Steel Corporation | Manufacturing | $529,845 | $533,260 | 30 | $7.3M | Research |
| Moffitt's Inc. | Wholesale Trade | $460,815 | $464,476 | 36 | $11.0M | Research |
| Pat Clemons INC | Retail Trade | $436,285 | $439,496 | 43 | $10.8M | Research |
| Pat Clemons, INC | Retail Trade | $407,502 | $412,403 | 36 | $9.4M | Research |
| Fisher Tracks Inc. | Construction | $404,300 | $407,737 | 40 | $15.0M | Research |
| Fisher Tracks, INC | Manufacturing | $371,781 | $374,208 | 41 | $11.4M | Research |
| Iowa High School Athletic Association | Educational Services | $358,657 | $360,719 | 24 | N/A | Research |
| Iowa High School Athletic Association | Educational Services | $319,332 | $321,071 | 18 | N/A | Research |
| Swan Creek Cabinetry INC | Manufacturing | $277,340 | $279,189 | 25 | $6.5M | Research |
| Boone Rehabilitation Services PC | Health Care and Social Assistance | $265,079 | $266,780 | 20 | <$5M | Research |
| Boone Rehabilitation Services PC | Health Care and Social Assistance | $265,079 | $266,471 | 29 | $7.8M | Research |
| Henkel & Associates PC | Professional, Scientific, and Technical Services | $256,645 | $261,372 | 17 | $5.4M | Research |
| Henkel & Associates PC | Professional, Scientific, and Technical Services | $256,645 | $263,781 | 17 | $5.4M | Research |
| Swan Creek Cabinetry, INC | Manufacturing | $254,447 | $255,931 | 32 | $8.4M | Research |
| Walters Sanitary Service INC | Administrative and Support and Waste Management | $246,773 | $248,164 | 23 | <$5M | Research |
| Pritchard Bros., Inc. | Construction | $199,750 | $201,320 | 17 | $5.4M | Research |
| Church of the Sacred Heart | Public Administration | $190,802 | $192,339 | 26 | N/A | Research |
| Jordan & Mahoney Law Firm PC | Professional, Scientific, and Technical Services | $176,200 | $176,978 | 10 | <$5M | Research |