| Modern Holdings, Inc. | Construction | $7,955,000 | $8,057,089 | 381 | $120.5M |  Research |
| Van Meter Inc. | Wholesale Trade | $7,413,400 | $7,505,244 | 496 | $151.9M | Research |
| Physicians' Clinic of Iowa, P.c. | Health Care and Social Assistance | $6,762,737 | $6,837,034 | 459 | $144.1M | Research |
| Shive-Hattery Group, Inc. | Professional, Scientific, and Technical Services | $6,058,800 | $6,128,186 | 397 | $123.1M | Research |
| Thomas L. Cardella & Associates, INC | Administrative and Support and Waste Management | $5,908,300 | $5,987,570 | 500 | $94.6M | Research |
| Four Oaks Family and Children's Services | Health Care and Social Assistance | $5,110,378 | $5,152,521 | 500 | $81.0M | Research |
| Igd Industries Holding Company, LLC | Other Services (except Public Administration) | $4,870,700 | $4,928,607 | 250 | $49.0M | Research |
| Don Hummer Trucking Corporatio | Transportation and Warehousing | $4,095,000 | $4,142,661 | 250 | $17.3M | Research |
| Involta, LLC | Professional, Scientific, and Technical Services | $3,977,000 | $4,004,729 | 260 | $26.9M | Research |
| Truenorth Companies Lc | Finance and Insurance | $3,897,000 | $3,948,852 | 323 | $166.0M | Research |
| Slb of Iowa, Lc | Accommodation and Food Services | $3,692,047 | $3,734,632 | 500 | $88.8M | Research |
| B G Brecke, INC | Construction | $3,561,187 | $3,602,932 | 154 | $48.7M | Research |
| Raining Rose, Inc. | Manufacturing | $3,537,400 | $3,578,201 | 250 | $248.0M | Research |
| Mount Mercy University | Educational Services | $3,384,327 | $3,420,709 | 335 | N/A | Research |
| Nrg Radio LLC A/k/a Nrg Media LLC | Information | $2,883,200 | $2,916,437 | 250 | $42.0M | Research |
| Folience INC | Management of Companies and Enterprises | $2,878,800 | $2,911,906 | 236 | $38.8M | Research |
| Highway Equipment Company | Manufacturing | $2,672,000 | $1,132,639 | 149 | $39.0M | Research |
| Iowa Fluid Power, Inc. | Manufacturing | $2,608,800 | $2,638,946 | 142 | $37.2M | Research |
| 7g Distributing, LLC | Wholesale Trade | $2,435,600 | $2,464,489 | 205 | $52.8M | Research |
| Engineered Seal Products, Inc. | Wholesale Trade | $2,335,328 | $2,362,184 | 163 | $49.9M | Research |
| Tanager Place | Health Care and Social Assistance | $2,286,000 | $2,312,226 | 236 | N/A | Research |
| Thompson Truck & Trailer, LLC | Wholesale Trade | $2,075,500 | $2,099,484 | 180 | $55.1M | Research |
| D.c. Taylor Co. | Construction | $2,049,000 | $2,072,848 | 132 | $43.5M | Research |
| Opn, Inc. | Professional, Scientific, and Technical Services | $2,022,500 | $2,046,264 | 107 | $33.2M | Research |
| Folience Inc. | Information | $2,000,000 | $2,014,500 | 212 | $35.6M | Research |