| Central Park Dentistry Charles City LLC | Health Care and Social Assistance | $196,205 | $197,126 | 17 | <$5M |  Research |
| Central Park Dentistry Charles City, LLC | Health Care and Social Assistance | $196,205 | $197,360 | 17 | <$5M | Research |
| Immaculate Conception Church | Other Services (except Public Administration) | $189,845 | $190,773 | 40 | N/A | Research |
| Immaculate Conception Church | Other Services (except Public Administration) | $189,845 | $191,242 | 40 | N/A | Research |
| Diversified Manufacturing Inc. | Manufacturing | $180,690 | $182,552 | 18 | <$5M | Research |
| Diversified Fastening Systems Inc. | Wholesale Trade | $174,545 | $176,344 | 14 | <$5M | Research |
| Swartzrock Implement Co., INC | Retail Trade | $168,537 | $169,744 | 16 | <$5M | Research |
| Enterprise Media, Inc. | Information | $162,130 | $163,220 | 24 | <$5M | Research |
| Thor Manufacturing LLC | Manufacturing | $158,100 | $159,088 | 15 | <$5M | Research |
| Thor Manufacturing LLC | Manufacturing | $156,245 | $133,230 | 19 | <$5M | Research |
| Lnj Industries, INC | Manufacturing | $147,760 | $148,722 | 13 | <$5M | Research |
| Anthony Bill Trucking | Transportation and Warehousing | $106,935 | $107,678 | 13 | <$5M | Research |
| Custom Wood Products INC | Manufacturing | $106,133 | $106,566 | 17 | <$5M | Research |
| Custom Wood Products Inc, | Manufacturing | $106,133 | $106,808 | 17 | <$5M | Research |
| Dabritosa INC | Accommodation and Food Services | $105,906 | $106,353 | 19 | <$5M | Research |
| Red Line Vending Inc. | Retail Trade | $104,208 | $104,975 | 15 | <$5M | Research |
| The Optometric Center of Charles City P.c. | Health Care and Social Assistance | $101,685 | $102,360 | 9 | <$5M | Research |
| Optometric Center of C. C. , P. C. | Health Care and Social Assistance | $101,600 | $102,613 | 10 | <$5M | Research |
| Avenue of the Saints Animal Hospital, P.l.l.c. | Professional, Scientific, and Technical Services | $89,700 | $90,228 | 21 | <$5M | Research |
| Triple J Ranch, Inc. | Accommodation and Food Services | $84,300 | $84,839 | 27 | <$5M | Research |
| Hometown Dental, Inc. | Health Care and Social Assistance | $84,067 | $84,727 | 10 | <$5M | Research |
| Unlimited Spirit Inc. | Accommodation and Food Services | $83,870 | $84,207 | 22 | <$5M | Research |
| Family Ymca of Charles City | Other Services (except Public Administration) | $82,225 | $82,830 | 42 | $8.0M | Research |
| Family Ymca of Charles City | Health Care and Social Assistance | $82,225 | $82,554 | 79 | $12.8M | Research |
| H B Corporation | Accommodation and Food Services | $81,394 | $81,808 | 14 | <$5M | Research |