| American Healthcare Management Services, LLC | Health Care and Social Assistance | $7,293,380 | $7,435,251 | 500 | $85.1M |  Research |
| Willis Cadillac Inc. | Retail Trade | $4,234,800 | $4,286,546 | 380 | $95.8M | Research |
| Mcclure Engineering Co | Professional, Scientific, and Technical Services | $3,316,900 | $3,360,112 | 187 | $23.1M | Research |
| Fawn Manufacturing, INC | Manufacturing | $2,061,500 | $2,088,554 | 256 | $67.0M | Research |
| Perfection Learning Corporation | Information | $1,615,872 | $1,628,976 | 105 | $17.6M | Research |
| Nationwide Office Cleaners,llc | Administrative and Support and Waste Management | $1,596,250 | $1,607,956 | 367 | $79.5M | Research |
| Dmhon, LLC | Retail Trade | $1,404,600 | $1,417,319 | 113 | $28.5M | Research |
| Iowa Digestive Disease Center | Health Care and Social Assistance | $1,385,103 | $1,395,083 | 74 | $23.2M | Research |
| Prairie Pella, Inc. | Wholesale Trade | $1,154,800 | $1,166,059 | 93 | $28.5M | Research |
| Midwest Professional Staffing LLC | Administrative and Support and Waste Management | $1,153,900 | $1,159,684 | 67 | $10.5M | Research |
| Gl Shh, LLC | Retail Trade | $1,090,880 | $1,101,986 | 82 | $20.7M | Research |
| K.r. Jones Enterprises, Inc. | Other Services (except Public Administration) | $1,087,700 | $1,098,396 | 88 | $17.2M | Research |
| A. J. S. of Des Moines, INC | Administrative and Support and Waste Management | $1,015,000 | $1,022,619 | 145 | $27.4M | Research |
| American Technology Consulting LLC | Administrative and Support and Waste Management | $972,700 | $985,412 | 46 | $7.2M | Research |
| American Prosthetics & Orthotics, Inc. | Manufacturing | $942,000 | $949,613 | 72 | $20.1M | Research |
| Iowa Diagnostic Imaging and Procedure Center | Health Care and Social Assistance | $863,627 | $872,023 | 69 | $16.0M | Research |
| Carrier Access, Inc. | Professional, Scientific, and Technical Services | $842,855 | $850,244 | 43 | <$5M | Research |
| Baker Interior Systems LLC | Construction | $812,500 | $815,615 | 43 | $14.7M | Research |
| Midwest Professional Staffing, LLC | Administrative and Support and Waste Management | $730,000 | $737,706 | 80 | $12.6M | Research |
| Priority Excavating, LLC | Construction | $680,695 | $686,663 | 32 | $9.9M | Research |
| Priority Excavating LLC | Construction | $673,684 | $681,544 | 32 | $9.9M | Research |
| Five Star, Fa, INC | Construction | $667,570 | $671,983 | 40 | $10.1M | Research |
| Five Star Fa Inc. | Retail Trade | $667,570 | $672,224 | 43 | $7.8M | Research |
| Valley Plbg. Co., Inc. | Construction | $665,262 | $671,619 | 65 | $20.6M | Research |
| Myriad Advisor Solutions | Other Services (except Public Administration) | $665,000 | $674,199 | 24 | <$5M | Research |