| Infinity Skin Care and Spa, LLC | Health Care and Social Assistance | $256,850 | $258,257 | 22 | $6.9M |  Research |
| Cantilever Hotels, L.l.c. | Accommodation and Food Services | $255,000 | $256,418 | 41 | $12.8M | Research |
| Vine Ventures INC | Accommodation and Food Services | $252,798 | $255,136 | 28 | <$5M | Research |
| Iowa City Westside Cafe Inc. | Accommodation and Food Services | $251,069 | $252,981 | 49 | $8.7M | Research |
| Mondo's New Union Brewery, Lc | Accommodation and Food Services | $249,900 | $251,851 | 32 | $5.7M | Research |
| Home Furnishings & Interior Design INC | Retail Trade | $238,150 | $239,195 | 23 | <$5M | Research |
| Home Furnishings & Interior Design INC | Retail Trade | $238,100 | $239,932 | 23 | <$5M | Research |
| Petsel Ventures, L.l.c. | Accommodation and Food Services | $231,600 | $233,852 | 68 | $12.1M | Research |
| The Iowa Children's Museum | Arts, Entertainment, and Recreation | $229,005 | $230,627 | 42 | N/A | Research |
| The Iowa Children's Museum | Arts, Entertainment, and Recreation | $229,005 | $230,220 | 22 | N/A | Research |
| Forever Green INC | Administrative and Support and Waste Management | $224,800 | $226,024 | 32 | $6.9M | Research |
| Brown Deer Place LLC | Health Care and Social Assistance | $206,400 | $208,000 | 32 | $5.4M | Research |
| D&w Enterprises INC | Accommodation and Food Services | $203,000 | $204,962 | 38 | $6.7M | Research |
| Iowa City Westside Cafe INC | Accommodation and Food Services | $202,600 | $205,331 | 1 | <$5M | Research |
| Handicare Day Care | Health Care and Social Assistance | $199,537 | $201,011 | 40 | $6.5M | Research |
| The Governor Group LLC | Real Estate and Rental and Leasing | $198,700 | $201,603 | 21 | <$5M | Research |
| Intrepid, LLC | Accommodation and Food Services | $194,800 | $195,883 | 29 | $9.1M | Research |
| Keith D Villhauer Enterprises INC | Transportation and Warehousing | $194,000 | $195,245 | 29 | <$5M | Research |
| Flip 3175 Inc. | Accommodation and Food Services | $189,067 | $191,283 | 20 | <$5M | Research |
| Carter and Associates, Inc. | Construction | $188,400 | $190,818 | 12 | <$5M | Research |
| Vetter's Inc. | Retail Trade | $187,500 | $190,385 | 18 | <$5M | Research |
| Cantilever Hotels, L.l.c. | Accommodation and Food Services | $182,100 | $183,205 | 41 | $12.8M | Research |
| Performance Health & Fitness L | Arts, Entertainment, and Recreation | $181,257 | $182,017 | 31 | <$5M | Research |
| Turf Masters Lawn and Landscape, LLC | Administrative and Support and Waste Management | $179,200 | $181,594 | 35 | $7.6M | Research |
| Mondo's New Union Brewery, Lc | Accommodation and Food Services | $178,500 | $180,449 | 52 | $9.2M | Research |