| Mccarthy-Bush Corporation | Construction | $9,016,600 | $8,192,964 | 465 | $529.8M |  Research |
| Palmer College Foundation | Educational Services | $7,358,700 | $6,349,666 | 421 | N/A | Research |
| Mississippi Valley Regional Blood Center | Health Care and Social Assistance | $7,152,507 | $7,185,289 | 500 | $115.9M | Research |
| Sears Manufacturing Company | Manufacturing | $6,914,338 | $6,955,446 | 500 | $130.9M | Research |
| Community Health Care, Inc. | Health Care and Social Assistance | $3,953,800 | $3,999,269 | 250 | N/A | Research |
| River Valley Cooperative | Wholesale Trade | $3,792,500 | $3,837,062 | 258 | $66.4M | Research |
| Cardiovascular Medicine, PC | Health Care and Social Assistance | $3,683,700 | $3,725,756 | 231 | $72.5M | Research |
| Rhythm City Casino LLC | Arts, Entertainment, and Recreation | $3,524,515 | $3,584,480 | 464 | $65.0M | Research |
| M.a. Ford Mfg Co. Inc. | Manufacturing | $3,401,000 | $3,433,593 | 232 | $60.7M | Research |
| Russell Construction Co., Inc. | Construction | $3,351,700 | $3,391,186 | 144 | $164.1M | Research |
| J.w. Koehler Electric Inc. | Construction | $3,094,567 | $3,129,381 | 78 | $25.7M | Research |
| Handicapped Development Center | Health Care and Social Assistance | $2,882,175 | $2,915,340 | 383 | N/A | Research |
| General Logistics, Inc. | Transportation and Warehousing | $2,513,289 | $2,547,009 | 147 | $29.6M | Research |
| Twin State Inc. | Retail Trade | $2,265,500 | $2,291,302 | 154 | $31.8M | Research |
| Mobotrex, Inc. | Construction | $2,204,500 | $2,229,791 | 165 | $54.3M | Research |
| Vera French Community Mental Health Center | Health Care and Social Assistance | $2,158,500 | $2,188,226 | 167 | N/A | Research |
| Estes Company, Ll | Construction | $2,028,700 | $2,052,030 | 127 | $144.7M | Research |
| Ragan Mechanical, Inc. | Construction | $1,999,875 | $2,025,928 | 57 | $18.0M | Research |
| Hahn Ready Mix Company | Manufacturing | $1,928,773 | $1,951,918 | 120 | $119.0M | Research |
| Advanced Steel Erectors, LLC | Administrative and Support and Waste Management | $1,862,638 | $1,890,474 | 105 | $16.5M | Research |
| Shaw Electric INC | Construction | $1,826,500 | $1,846,997 | 123 | $40.5M | Research |
| S.j. Smith Company INC | Wholesale Trade | $1,825,363 | $1,840,575 | 122 | $31.4M | Research |
| Midwest Thermal Insulation, Inc. | Administrative and Support and Waste Management | $1,804,700 | $1,814,776 | 115 | $21.8M | Research |
| Seither and Cherry Quad Cities Inc. | Other Services (except Public Administration) | $1,717,500 | $1,729,311 | 68 | $14.6M | Research |
| Kahl Home for the Aged and Infirm | Health Care and Social Assistance | $1,699,293 | $1,717,869 | 214 | N/A | Research |