| Winneshiek Medical Center | Health Care and Social Assistance | $6,558,287 | $5,808,907 | 485 | N/A |  Research |
| Bruening Rock Products, Inc. | Mining, Quarrying, and Oil and Gas Extraction | $4,785,300 | $4,846,657 | 223 | $13.5M | Research |
| Iowa Rotocast Plastics, Inc. | Manufacturing | $2,012,935 | $2,036,373 | 172 | $170.6M | Research |
| Iowa Rotocast Plastics Inc. | Manufacturing | $2,000,000 | $2,008,110 | 147 | $145.8M | Research |
| Deco Products Company, Lllp | Manufacturing | $1,750,000 | $1,761,764 | 198 | $51.8M | Research |
| Northeast Iowa Community Action Corp | Public Administration | $1,236,485 | $807,546 | 165 | N/A | Research |
| Toppling Goliath Brewing Co. | Manufacturing | $1,143,498 | $1,155,120 | 78 | $27.5M | Research |
| Jb Holland Construction, Inc. | Construction | $922,307 | $933,401 | 70 | $21.7M | Research |
| Aase Haugen Homes, Inc. | Health Care and Social Assistance | $786,708 | $791,406 | 137 | N/A | Research |
| Wicks Construction Inc. | Construction | $745,575 | $749,738 | 73 | $16.9M | Research |
| Wicks Construction INC | Construction | $745,575 | $747,791 | 60 | $13.9M | Research |
| Aase Haugen Homes Inc. | Health Care and Social Assistance | $722,145 | $726,280 | 99 | N/A | Research |
| Legacy Travel Group | Transportation and Warehousing | $698,251 | $701,770 | 46 | $17.7M | Research |
| Hack, Nelson & Co. P.c. | Professional, Scientific, and Technical Services | $679,710 | $688,187 | 45 | $14.3M | Research |
| Legacy Travel Group | Transportation and Warehousing | $630,898 | $634,536 | 68 | $26.1M | Research |
| Opportunity Homes INC | Health Care and Social Assistance | $548,933 | $551,875 | 55 | N/A | Research |
| Olson Explosives, INC | Mining, Quarrying, and Oil and Gas Extraction | $529,762 | $533,739 | 31 | <$5M | Research |
| Olson Explosives, INC | Mining, Quarrying, and Oil and Gas Extraction | $529,762 | $531,867 | 33 | <$5M | Research |
| Windridge Implements, LLC | Wholesale Trade | $501,468 | $504,312 | 43 | $13.2M | Research |
| Oneota Riverview Care Facility, Inc. | Health Care and Social Assistance | $450,855 | $454,869 | 52 | N/A | Research |
| Seed Savers Exchange Inc. | Wholesale Trade | $432,367 | $434,800 | 53 | N/A | Research |
| Barthell Oes Home | Health Care and Social Assistance | $413,832 | $416,259 | 89 | N/A | Research |
| Decorah Chevrolet Cadillac Inc. | Retail Trade | $368,741 | $370,155 | 28 | $7.1M | Research |
| Perry Novak Electric, INC | Construction | $355,600 | $357,597 | 25 | $8.2M | Research |
| Casper Plbg & Htg Inc. | Construction | $343,440 | $345,338 | 18 | $5.7M | Research |